• Immunofluorescence analysis of L929 cells using MMP25 Rabbit polyclonal antibody (STJ118331) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using MMP25 Rabbit polyclonal antibody (STJ118331) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MMP25 in paraffin-embedded mouse spleen using MMP25 Rabbit polyclonal antibody (STJ118331) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MMP25 in paraffin-embedded human lung cancer using MMP25 Rabbit polyclonal antibody (STJ118331) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MMP25 in paraffin-embedded rat spleen using MMP25 Rabbit polyclonal antibody (STJ118331) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MMP25 Rabbit polyclonal antibody (STJ118331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MMP25 antibody (321-539) (STJ118331)

SKU:
STJ118331

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP25 (321-539) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP25
Gene ID: 64386
Uniprot ID: MMP25_HUMAN
Immunogen Region: 321-539
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 321-539 of human MMP25 (NP_071913.1).
Immunogen Sequence: FDAIANIRGETFFFKGPWFW RLQPSGQLVSPRPARLHRFW EGLPAQVRVVQAAYARHRDG RILLFSGPQFWVFQDRQLEG GARPLTELGLPPGEEVDAVF SWPQNGKTYLVRGRQYWRYD EAAARPDPGYPRDLSLWEGA PPSPDDVTVSNAGDTYFFKG AHYWRFPKNSIKTEPDAPQP MGPNWLDCPAPSSGPRAPRP PKATPVSETCDCQCELNQA
Tissue Specificity Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function May activate progelatinase A.
Protein Name Matrix Metalloproteinase-25
Mmp-25
Leukolysin
Membrane-Type Matrix Metalloproteinase 6
Mt-Mmp 6
Mtmmp6
Membrane-Type-6 Matrix Metalloproteinase
Mt6-Mmp
Mt6mmp
Database Links Reactome: R-HSA-1592389
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Metalloproteinase-25 antibody
Anti-Mmp-25 antibody
Anti-Leukolysin antibody
Anti-Membrane-Type Matrix Metalloproteinase 6 antibody
Anti-Mt-Mmp 6 antibody
Anti-Mtmmp6 antibody
Anti-Membrane-Type-6 Matrix Metalloproteinase antibody
Anti-Mt6-Mmp antibody
Anti-Mt6mmp antibody
Anti-MMP25 antibody
Anti-MMP20 antibody
Anti-MMPL1 antibody
Anti-MT6MMP antibody

Information sourced from Uniprot.org

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