• Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-R2 Rabbit polyclonal antibody (STJ24010). Blue: DAPI for nuclear staining.
  • Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R2 antibody (STJ24010) at 1:1000 dilution.
  • Western blot analysis of various lysates using MonoMethyl-Histone H3-R2 Rabbit polyclonal antibody (STJ24010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-Mono-Methyl-Histone H3-R2 antibody (STJ24010)

SKU:
STJ24010

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mono-Methyl-Histone H3-R2 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic monomethylated peptide around R2 of human histone H3 (NP_003520.1).
Immunogen Sequence: MARTK
Background Enables double-stranded telomeric DNA binding activity; identical protein binding activity; and transcription cis-regulatory region binding activity. Involved in positive regulation of transcription, DNA-templated and telomere maintenance via telomere lengthening. Located in chromosome, telomeric region.

Information sourced from Uniprot.org

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