• Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23966) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23966) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23966) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded mouse kidney using MonoMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23966) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded human breast using MonoMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23966) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MonoMethyl-Histone H3-R17 in paraffin-embedded rat lung using MonoMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23966) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R17 antibody (STJ23966).
  • Western blot analysis of lysates from C6 cells, using MonoMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23966) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Mono-Methyl-Histone H3-R17 antibody (STJ23966)

SKU:
STJ23966

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mono-Methyl-Histone H3-R17 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic monomethylated peptide around R17 of human histone H3 (NP_003520.1).
Immunogen Sequence: APRKQ
Background Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.

Information sourced from Uniprot.org

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