Immunocytochemistry Antibodies | St John's Laboratory

Immunofluorescence analysis of MCF-7 cells using RUNX3 antibody (STJ29442). Blue: DAPI for nuclear staining.

Anti-RUNX3 antibody (286-415) (STJ29442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-RUSC1 antibody (1-320) (STJ117612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using RUVBL1/TIP49A/PONTIN antibody (STJ116128) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RUVBL1 antibody (1-214) (STJ116128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A549 cells using RUVBL1/TIP49A/PONTIN antibody (STJ28290). Blue: DAPI for nuclear staining.

Anti-RUVBL1 antibody (1-240) (STJ28290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR Alpha antibody (STJ11101642) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RXRA antibody (1-100) [S2MR] (STJ11101642)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunocytochemistry analysis of RYK (green) in hela using RYK antibody, and DAPI (blue)

Anti-RYK antibody (575-589 aa) [R03-4E1] (STJA0012167)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£264.50

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Western blot analysis of lysates from Mouse heart using RYR2 Rabbit pAb (STJ11107062) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time:90s.

Anti-RYR2 antibody (1411-1510 aa) (STJ11107062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded mouse heart using RYR2 Rabbit polyclonal antibody (STJ25428) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RYR2 antibody (4932-4948) (STJ25428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using S100A10 antibody (STJ116864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-S100A10 antibody (1-97) (STJ116864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using S100A10 antibody (STJ25429) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-S100A10 antibody (1-97) (STJ25429)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£112.50 - £524.50

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Immunofluorescence analysis of MCF7 cells using S100A11 Rabbit polyclonal antibody (STJ11104605) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-S100A11 antibody (1-105) (STJ11104605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of THP-1 cells using 3 Mu g S100A11 antibody (STJ27439). Western blot was performed from the immunoprecipitate using S100A11 antibody (STJ27439) at a dilution of 1:1000.

Anti-S100A11 antibody (1-105) (STJ27439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of 293F, using S100A12 antibody (STJ27281) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-S100A12 antibody (1-92) (STJ27281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using S100A14 Rabbit polyclonal antibody (STJ112430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-S100A14 antibody (1-104) (STJ112430)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using FSP1/S100A4 Rabbit polyclonal antibody (STJ11103860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-S100A4 antibody (1-101) (STJ11103860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of FSP1/S100A4 in paraffin-embedded human liver using FSP1/S100A4 Rabbit polyclonal antibody (STJ11104557) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-S100A4 antibody (1-101) (STJ11104557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Confocal imaging of paraffin-embedded Human colon using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-S100A4 antibody (2-101) [S6MR] (STJ11101646)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from U-251 MG cells using S100A6 Rabbit pAb (STJ11106830) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-S100A6 antibody (1-90 aa) (STJ11106830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-S100A6 antibody (1-90) (STJ25432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Confocal imaging of C2C12 cells using S100A6 Rabbit monoclonal antibody (STJ11101874, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-S100A6 antibody (1-90) [S4MR] (STJ11101874)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Confocal immunofluorescence analysis of U-2 OS cells using S100A8 Polyclonal Antibody (STJ117510) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-S100A8 antibody (1-93) (STJ117510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded human skin cancer using S100A8 Rabbit polyclonal antibody (STJ25434) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-S100A8 antibody (1-93) (STJ25434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-S100A9 antibody (1-113 aa) [SRM] (STJ11107246)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from Mouse spleen using S100A9 Rabbit pAb (STJ111884) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-S100A9 antibody (20-114) (STJ111884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g S100B antibody (STJ29811). Western blot was performed from the immunoprecipitate using S100B antibody (STJ29811) at a dilution of 1:500.

Anti-S100B antibody (1-92) (STJ29811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Rat brain, using S100B rabbit monoclonal antibody (STJ11101645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-S100B antibody (1-92) [S5MR] (STJ11101645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using S100P Rabbit pAb (STJ116980) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-S100P antibody (1-95) (STJ116980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-S100P antibody (C-Term) (STJ502848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£466.50

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Anti-S100P antibody (C-Term) {Biotin} (STJ502851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-S100P antibody (C-Term) {FITC} (STJ502852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Western blot with Anti-S100P Antibody (STJ502846) with S100B peptide. Antibody at 1:500 dilution in buffer. 15% discontinuous gel.

Anti-S100P antibody (Full Length) (STJ502846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£466.50

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Anti-S100P antibody (Full Length) {Biotin} (STJ502849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-S100P antibody (Full Length) {FITC} (STJ502850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-S100P antibody (N-Term) (STJ502847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£466.50

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Anti-S100P antibody (N-Term) {Biotin} (STJ502853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-S100P antibody (N-Term) {FITC} (STJ502854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Rat

£528.50

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Western blot analysis of various lysates using SAFB Rabbit pAb (STJ110236) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-SAFB antibody (1-200) (STJ110236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using SAFB antibody (STJ118167) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SAFB antibody (300-400) (STJ118167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot detection of SAFB1 in HelaNE, A431, MDA-MB-468, Breast cancer, ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa.

Anti-SAFB antibody [2E8-E2-G6] (STJ99122)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates using Sall4 Rabbit pAb (STJ29204) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 90s.

Anti-SALL4 antibody (1-220) (STJ29204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded rat testis using Sall4 Rabbit polyclonal antibody (STJ118633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SALL4 antibody (853-1053) (STJ118633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of SAMD9 in A549 lysates using SAMD9 antibody.

Anti-SAMD9 antibody (84-189 aa) [R09-5B4] (STJA0011932)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£264.50

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Western blot analysis of various lysates, using SARDH Rabbit pAb (STJ113655) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 40s.

Anti-SARDH antibody (300-560) (STJ113655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-SARM1 antibody (Full Length) (STJA0004904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£454.50

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Immunofluorescence analysis of U-2 OS cells using SARNP Rabbit polyclonal antibody (STJ115656) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SARNP antibody (1-210) (STJ115656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of MCF7 cells using SARS antibody (STJ28816). Blue: DAPI for nuclear staining.

Anti-SARS antibody (1-300) (STJ28816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein antibody (STJ11101492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SARS-CoV-2 Nucleoprotein antibody (1-420) (STJ11101492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/IP/ELISA

Reactivity :

SARS-CoV-2

£146.50 - £642.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike antibody (STJ11101794). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike antibody (STJ11101794) at a dilution of 1:1000.

Anti-SARS-CoV-2 Spike antibody (600-700) (STJ11101794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/IP/ELISA

Reactivity :

SARS-CoV-2

£112.50 - £524.50

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