Western blot analysis of extracts of HeLa cells, using Monoamine Oxidase B (Monoamine Oxidase B (MAOB) ) antibody (STJ24454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAOB antibody (400-490) (STJ24454)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ24454
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General Information

Short DescriptionRabbit polyclonal MAOB (400-490) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAOB
Gene ID4129
Uniprot IDAOFB_HUMAN
Immunogen Region400-490
Immunogen SequenceTYFPPGILTQYGRVLRQPVD RIYFAGTETATHWSGYMEGA VEAGERAAREILHAMGKIPE DEIWQSEPESVDVPAQPITT TFLERHLPSVP
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 400-490 of human Monoamine Oxidase B (MAOB) (NP_000889.3).

Additional Info

Function Catalyzes the oxidative deamination of primary and some secondary amines such as neurotransmitters, and exogenous amines including the tertiary amine, neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide and participates in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Preferentially degrades benzylamine and phenylethylamine.
Protein Name Amine Oxidase Flavin-Containing B
Monoamine Oxidase Type B
Mao-B
Database Links Reactome: R-HSA-141333
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Amine Oxidase Flavin-Containing B antibody
Anti-Monoamine Oxidase Type B antibody
Anti-Mao-B antibody
Anti-MAOB antibody

Information sourced from Uniprot.org

Citations

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