• Anti-MAP1LC3B antibody (2-107) [S4MR] (STJ11101754)
  • Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KD Validated] LC3B Rabbit monoclonal antibody (STJ11101754). Western blot was performed from the immunoprecipitate using LC3B antibody (STJ11101754) at a dilution of 1:1000.
  • Confocal imaging of HeLa cells (treated with chloroquine) and HeLa cells (untreated) using [KD Validated] LC3B Rabbit monoclonal antibody (STJ11101754, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of LC3B in paraffin-embedded rat brain using [KD Validated] LC3B Rabbit monoclonal antibody (STJ11101754) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LC3B in paraffin-embedded human brain using [KD Validated] LC3B Rabbit monoclonal antibody (STJ11101754) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and LKB1 knockdown (KD) 293T cells, using [KD Validated] LC3B Rabbit monoclonal antibody (STJ11101754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit monoclonal antibody (STJ11101754) at 1:1000 dilution. 293T, C6 and NIH/3T3 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MAP1LC3B antibody (2-107) [S4MR] (STJ11101754)

SKU:
STJ11101754

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LC3B (2-107) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP1LC3B
Gene ID: 81631
Uniprot ID: MLP3B_HUMAN
Immunogen Region: 2-107
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B (Q9GZQ8).
Immunogen Sequence: PSEKTFKQRRTFEQRVEDVR LIREQHPTKIPVIIERYKGE KQLPVLDKTKFLVPDHVNMS ELIKIIRRRLQLNANQAFFL LVNGHSMVSVSTPISEVYES EKDEDG
Tissue Specificity Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
Post Translational Modifications The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation by PKA inhibits conjugation of phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy.
Protein Name Microtubule-Associated Proteins 1a/1b Light Chain 3b
Autophagy-Related Protein Lc3 B
Autophagy-Related Ubiquitin-Like Modifier Lc3 B
Map1 Light Chain 3-Like Protein 2
Map1a/Map1b Light Chain 3 B
Map1a/Map1b Lc3 B
Microtubule-Associated Protein 1 Light Chain 3 Beta
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5205685
Reactome: R-HSA-8854214
Reactome: R-HSA-8934903
Reactome: R-HSA-9664873
Reactome: R-HSA-9679504
Reactome: R-HSA-9694676
Reactome: R-HSA-9754560
Reactome: R-HSA-9755511
Cellular Localisation Cytoplasmic Vesicle
Autophagosome Membrane
Lipid-Anchor
Endomembrane System
Mitochondrion Membrane
Cytoplasm
Cytoskeleton
Lc3-Ii Binds To The Autophagic Membranes
Lc3-Ii Localizes With The Mitochondrial Inner Membrane During Parkin-Mediated Mitophagy
Localizes Also To Discrete Punctae Along The Ciliary Axoneme
Alternative Antibody Names Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3b antibody
Anti-Autophagy-Related Protein Lc3 B antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 B antibody
Anti-Map1 Light Chain 3-Like Protein 2 antibody
Anti-Map1a/Map1b Light Chain 3 B antibody
Anti-Map1a/Map1b Lc3 B antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Beta antibody
Anti-MAP1LC3B antibody
Anti-MAP1ALC3 antibody

Information sourced from Uniprot.org

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