• Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti-MAP2 (STJA0003676, red, 1:10,000) , and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
  • Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
  • Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (STJA0003676, red, 1:5000) and Anti-FOX3 ( cat. STJA0003841, green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
  • Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (STJA0003676, 1:1000, red) and anti-synapsin (STJA0003781, 1:1000, green).
  • Costaining of the primary brain cells isolated from 18-day embryonic rats (E18). Neurons stained with Alpha-MAP2 (green). Copurified astrocytes stained with Alpha-GFAP (red). Image from publication CC-BY-4. 0. PMID: 35652638
  • Immunostaining of rat hippocampus showing specific immunolabeling of MAP2 (STJA0003676, red, 1:1000) , Tau-1 (green) , and nuclear DNA labeled with DAPI (blue). Photo courtesy of the Miriam Zamorano, University of Texas Health Science Center at Houston.

Anti-MAP2 antibody (STJA0003676)

SKU:
STJA0003676-100

£394.50
Shipping:
Free Shipping
Current Stock:
Host: Chicken
Applications: WB/IHC/ICC
Reactivity: Bovine/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Chicken polyclonal antibody anti-MAP2 is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgY
Formulation: Total IgY fraction in PBS + 10 mM Sodium Azide.
Purification: This antibody was total igy fraction.
Dilution Range: WB 1:20000
IHC 1:2500-1:10, 000
ICC 1:1000-1:20, 000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2
Gene ID: 4133
Uniprot ID: MTAP2_HUMAN
Immunogen: recombinant bovine MAP2 protein expressed in and purification from E. Coli.
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Protein Name Microtubule-Associated Protein 2
Map-2
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Alternative Antibody Names Anti-Microtubule-Associated Protein 2 antibody
Anti-Map-2 antibody
Anti-MAP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance