• Immunofluorescence analysis of HeLa cells using MAGE-1/MAGE1A antibody (STJ27423). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human stomach cancer using MAGE-1/MAGE1A antibody (STJ27423) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using MAGE-1/MAGE1A antibody (STJ27423) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MAGE-1/MAGE1A antibody (STJ27423) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of 22Rv1 cells, using MAGE-1/MAGE1A antibody (STJ27423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAGEA1 antibody (1-309) (STJ27423)

SKU:
STJ27423

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAGEA1 (1-309) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAGEA1
Gene ID: 4100
Uniprot ID: MAGA1_HUMAN
Immunogen Region: 1-309
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGE-1/MAGE1A (NP_004979.3).
Immunogen Sequence: MSLEQRSLHCKPEEALEAQQ EALGLVCVQAATSSSSPLVL GTLEEVPTAGSTDPPQSPQG ASAFPTTINFTRQRQPSEGS SSREEEGPSTSCILESLFRA VITKKVADLVGFLLLKYRAR EPVTKAEMLESVIKNYKHCF PEIFGKASESLQLVFGIDVK EADPTGHSYVLVTCLGLSYD GLLGDNQIMPKTGFLIIVLV MIAMEGGHAPEEEIWEELSV MEVYDGREHSAYGEPRKLL
Tissue Specificity Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.
Function May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Protein Name Melanoma-Associated Antigen 1
Antigen Mz2-E
Cancer/Testis Antigen 1.1
Ct1.1
Mage-1 Antigen
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Melanoma-Associated Antigen 1 antibody
Anti-Antigen Mz2-E antibody
Anti-Cancer/Testis Antigen 1.1 antibody
Anti-Ct1.1 antibody
Anti-Mage-1 Antigen antibody
Anti-MAGEA1 antibody
Anti-MAGE1 antibody
Anti-MAGE1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance