• Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (STJ27585) at 1:500 dilution. 293T cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. C6 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of various lysates, using LC3A/LC3B rabbit polyclonal antibody (STJ27585) at 1:2000 dilution. 293T, NIH/3T3 and C6 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using LC3A/LC3B rabbit polyclonal antibody (STJ27585) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using LC3A/LC3B rabbit polyclonal antibody (STJ27585) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using LC3A/LC3B rabbit polyclonal antibody (STJ27585) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using LC3A/LC3B rabbit polyclonal antibody (STJ27585) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of 293T Treated with Chloroquine and 293T cells using LC3A/LC3B rabbit polyclonal antibody (STJ27585) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAP1LC3A/MAP1LC3B antibody (1-60) (STJ27585)

SKU:
STJ27585

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LC3A/LC3B (1-60) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-60
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-60 of human LC3A (NP_115903.1).
Immunogen Sequence: MPSDRPFKQRRSFADRCKEV QQIRDQHPSKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM
Background Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Information sourced from Uniprot.org

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