• Western blot analysis of extracts of various cell lines, using MAGEA6 antibody (STJ116318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using MAGEA6 antibody (STJ116318) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using MAGEA6 antibody (STJ116318) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MAGEA6 antibody (STJ116318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAGEA6 antibody (75-314) (STJ116318)

SKU:
STJ116318

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAGEA6 (75-314) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAGEA6
Gene ID: 4105
Uniprot ID: MAGA6_HUMAN
Immunogen Region: 75-314
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 75-314 of human MAGEA6 (NP_005354.1).
Immunogen Sequence: MNYPLWSQSYEDSSNQEEEG PSTFPDLESEFQAALSRKVA KLVHFLLLKYRAREPVTKAE MLGSVVGNWQYFFPVIFSKA SDSLQLVFGIELMEVDPIGH VYIFATCLGLSYDGLLGDNQ IMPKTGFLIIILAIIAKEGD CAPEEKIWEELSVLEVFEGR EDSIFGDPKKLLTQYFVQEN YLEYRQVPGSDPACYEFLWG PRALIETSYVKVLHHMVKIS GGPRISYPLLHEWALREGE
Tissue Specificity Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
Post Translational Modifications Ubiquitinated by the DCX(DCAF12) complex specifically recognizes the diglutamate (Glu-Glu) at the C-terminus, leading to its degradation.
Function Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a as repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.
Protein Name Melanoma-Associated Antigen 6
Cancer/Testis Antigen 1.6
Ct1.6
Mage-6 Antigen
Mage3b Antigen
Alternative Antibody Names Anti-Melanoma-Associated Antigen 6 antibody
Anti-Cancer/Testis Antigen 1.6 antibody
Anti-Ct1.6 antibody
Anti-Mage-6 Antigen antibody
Anti-Mage3b Antigen antibody
Anti-MAGEA6 antibody
Anti-MAGE6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance