Primary Antibodies | St John's Laboratory

Immunofluorescence analysis of U2OS cells using PTEN Rabbit polyclonal antibody (STJ29771) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PTEN antibody (1-403) (STJ29771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human placenta using SPON2 antibody (STJ113975) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SPON2 antibody (27-150) (STJ113975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit pAb (STJ113725) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PIK3R1 antibody (240-724) (STJ113725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ73642 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-EPHB6 antibody (Internal) (STJ73642)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ73638 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PRKAB2 antibody (Internal) (STJ73638)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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STJ73631 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-LIF antibody (Internal) (STJ73631)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IHC

Reactivity :

Human

£312.50

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STJ73622 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PROM1/CD133 antibody (310-322) (STJ73622)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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STJ73621 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and secreted staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TNFSF11/OPGL antibody (Internal) (STJ73621)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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STJ73610 (0. 1µg/ml) staining of Brain of fetal Mouse (A) + peptide (B) , Mouse (C) + peptide (D) , Human (E) + peptide (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Nat8l-Mouse antibody (211-223) (STJ73610)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ73609 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-CTGF antibody (111-123) (STJ73609)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ73601 (0. 1µg/ml) staining of Human Cerebellum (A) + Peptide (B) , NIH3T3 (C) and negative Control Adrenal Gland (D) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-KCNN4/KCa3.1 antibody (Internal) (STJ73601)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ73599 (0. 3µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-OCIL antibody (Internal) (STJ73599)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human/Dog/Cow

£312.50

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STJ73596 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Hyaluronidase 1 antibody (Internal) (STJ73596)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ73588 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Pituitary tumor-transforming 1/Securin antibody (Internal) (STJ73588)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human/Mouse/Dog

£312.50

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STJ73487 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-TRPC1 antibody (Internal) (STJ73487)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ73486 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-PPP1R12A antibody (C-Term) (STJ73486)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human

£312.50

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STJ73481 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-DDX58 antibody (C-Term) (STJ73481)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse

£312.50

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STJ73426 (0. 1µg/ml) staining of K562 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BMI1 antibody (237-251) (STJ73426)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Dog/Pig

£312.50

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STJ73403 (0. 03µg/ml) staining of Human Platelets lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-tropomyosin 4/TPM4 antibody (C-Term) (STJ73403)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ73250 (1µg/ml) staining of HepG2 (A) and MCF7 (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA5/GRP78 antibody (Internal) (STJ73250)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human

£312.50

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STJ73249 (0. 1µg/ml) staining of HepG2 (A) , HeLa (B) and MCF7 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA5/GRP78 antibody (118-131) (STJ73249)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/WB

Reactivity :

Human

£312.50

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STJ73246 (0. 03µg/ml) staining of Human Heart (A) , Kidney (B) and Liver (C) lysate and in Pig Heart (D) , Kidney (E) and Liver (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSP60/HSPD1 antibody (333-344) (STJ73246)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Dog/Pig/Cow

£312.50

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STJ73107 (1µg/ml) staining of Human Heart (A) and Kidney (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-BMP1 antibody (284-296) (STJ73107)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ73053 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-contactin 1 antibody (585-870) (STJ73053)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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STJ73035 (0. 3µg/ml) staining of Human Olfactory bulb lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HOXC6-Isoform 1 antibody (Internal) (STJ73035)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72990 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Pycard antibody (Internal) (STJ72990)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72955 (0. 1µg/ml) staining of Human (A) , (0. 03µg/ml) Mouse (B) , (0. 1µg/ml) Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-TNNI3 antibody (117-127) (STJ72955)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72942 (0. 5µg/ml) staining of MEF lysates (35µg protein in RIPA buffer) , from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.

Anti-MK2/MAPKAPK2 antibody (Internal) (STJ72942)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IP/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ72920 (0. 03µg/ml) staining of Human Frontal cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AP2A1 antibody (706-727) (STJ72920)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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STJ72890 (0. 03µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-calbindin D28 antibody (Internal) (STJ72890)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Rat/Dog/Pig/Cow

£312.50

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STJ72849 (0. 05µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PCNA antibody (111-122) (STJ72849)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72848 (0. 01µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PCNA antibody (Internal) (STJ72848)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72813 (2µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PRMT2 antibody (Internal) (STJ72813)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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STJ72805 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-RIF1 antibody (C-Term) (STJ72805)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Dog/Pig/Cow

£312.50

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STJ72791 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-USP6 antibody (142-155) (STJ72791)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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STJ72753 (1µg/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TBP/Transcription factor IID antibody (39-50) (STJ72753)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72748 (0. 3µg/ml) staining of K562 (A) , Molt4 (B) and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-STAT5A antibody (C-Term) (STJ72748)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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STJ72745 (0. 1µg/ml) staining of Human Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-LCK antibody (39-52) (STJ72745)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72706 (1µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Slc5a1-Rat antibody (Internal) (STJ72706)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Rat

£312.50

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STJ72698 (0. 5µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CDH1 antibody (662-675) (STJ72698)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Rat/Dog/Pig

£312.50

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STJ72690 (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NPR3 antibody (520-533) (STJ72690)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72610 (0. 3µg/ml) staining of Human Skeletal muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-IDH3B antibody (33-46) (STJ72610)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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STJ72608 (0. 1µg/ml) staining of Human Lymph Nodes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IDH3A antibody (C-Term) (STJ72608)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72599 (0. 3µg/ml) staining of Human Cerebellum (A) , Mouse Brain (B) , Rat Brain (C) and Pig Brain (D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-MNSOD antibody (119-130) (STJ72599)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow/Zebrafish

£312.50

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STJ72597 (0. 3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Sirtuin 3-Mouse antibody (Internal) (STJ72597)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Pig

£312.50

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STJ72509 (0. 1µg/ml) staining of Human Colon (A) , (0. 03ug/ml) Liver (B) and (0. 01ug/ml) Testes (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-RORC antibody (200-212) (STJ72509)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Dog/Cow

£312.50

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STJ72505 (0. 1µg/ml) staining of Mouse (A) and Rat (B) Testis lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-GNAQ/ALPHA-q antibody (162-175) (STJ72505)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ72503 (0. 3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD28 antibody (Internal) (STJ72503)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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