• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MED1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from Jurkat cells, using MED1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using TRAP220 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED1 antibody (641-690 aa) (STJ96089)

SKU:
STJ96089

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mediator of RNA polymerase II transcription subunit 1 (641-690 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MED1
Gene ID: 5469
Uniprot ID: MED1_HUMAN
Immunogen Region: 641-690 aa
Specificity: TRAP220 Polyclonal Antibody detects endogenous levels of TRAP220 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MED1 at the amino acid range 641-690
Post Translational Modifications Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylation increases its interaction with PSIP1.
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.
Protein Name Mediator Of Rna Polymerase Ii Transcription Subunit 1
Activator-Recruited Cofactor 205 Kda Component
Arc205
Mediator Complex Subunit 1
Peroxisome Proliferator-Activated Receptor-Binding Protein
Pbp
Ppar-Binding Protein
Thyroid Hormone Receptor-Associated Protein Complex 220 Kda Component
Trap220
Thyroid Receptor-Interacting Protein 2
Tr-Interacting Protein 2
Trip-2
Vitamin D Receptor-Interacting Protein Complex Component Drip205
P53 Regulatory Protein Rb18a
Database Links Reactome: R-HSA-1368082
Reactome: R-HSA-1368108
Reactome: R-HSA-1989781
Reactome: R-HSA-212436
Reactome: R-HSA-2151201
Reactome: R-HSA-2426168
Reactome: R-HSA-381340
Reactome: R-HSA-383280
Reactome: R-HSA-400206
Reactome: R-HSA-400253
Reactome: R-HSA-9018519
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
A Subset Of The Protein May Enter The Nucleolus Subsequent To Phosphorylation By Mapk1 Or Mapk3
Alternative Antibody Names Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 1 antibody
Anti-Activator-Recruited Cofactor 205 Kda Component antibody
Anti-Arc205 antibody
Anti-Mediator Complex Subunit 1 antibody
Anti-Peroxisome Proliferator-Activated Receptor-Binding Protein antibody
Anti-Pbp antibody
Anti-Ppar-Binding Protein antibody
Anti-Thyroid Hormone Receptor-Associated Protein Complex 220 Kda Component antibody
Anti-Trap220 antibody
Anti-Thyroid Receptor-Interacting Protein 2 antibody
Anti-Tr-Interacting Protein 2 antibody
Anti-Trip-2 antibody
Anti-Vitamin D Receptor-Interacting Protein Complex Component Drip205 antibody
Anti-P53 Regulatory Protein Rb18a antibody
Anti-MED1 antibody
Anti-ARC205 antibody
Anti-CRSP1 antibody
Anti-CRSP200 antibody
Anti-DRIP205 antibody
Anti-DRIP230 antibody
Anti-PBP antibody
Anti-PPARBP antibody
Anti-PPARGBP antibody
Anti-RB18A antibody
Anti-TRAP220 antibody
Anti-TRIP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance