Anti-TRAF3 antibody (240-289 aa) (STJ96083)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TRAF3 at the amino acid range 240-289
General Information
| Short Description | Rabbit polyclonal anti-TNF receptor-associated factor 3 (240-289 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TRAF3 |
| Gene ID | 7187 |
| Uniprot ID | TRAF3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TRAF3 at the amino acid range 240-289 |
| Immunogen Region | 240-289 aa |
| Specificity | TRAF3 Polyclonal Antibody detects endogenous levels of TRAF3 protein. |
Additional Info
| Function | Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus controls a lot of biological processes in both immune and non-immune cell types. In TLR and RLR signaling pathways, acts as an E3 ubiquitin ligase promoting the synthesis of 'Lys-63'-linked polyubiquitin chains on several substrates such as ASC that lead to the activation of the type I interferon response or the inflammasome. Following the activation of certain TLRs such as TLR4, acts as a negative NF-kappa-B regulator, possibly to avoid unregulated inflammatory response, and its degradation via 'Lys-48'-linked polyubiquitination is required for MAPK activation and production of inflammatory cytokines. Alternatively, when TLR4 orchestrates bacterial expulsion, TRAF3 undergoes 'Lys-33'-linked polyubiquitination and subsequently binds to RALGDS, mobilizing the exocyst complex to rapidly expel intracellular bacteria back for clearance. Also acts as a constitutive negative regulator of the alternative NF-kappa-B pathway, which controls B-cell survival and lymphoid organ development. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. |
| Protein Name | Tnf Receptor-Associated Factor 3Cd40 Receptor-Associated Factor 1Craf1Cd40-Binding ProteinCd40bpLmp1-Associated Protein 1Lap1Ring-Type E3 Ubiquitin Transferase Traf3 |
| Database Links | Reactome: R-HSA-5602571Reactome: R-HSA-5668541Reactome: R-HSA-5676594Reactome: R-HSA-5689896Reactome: R-HSA-9013973Reactome: R-HSA-918233Reactome: R-HSA-936440Reactome: R-HSA-936964Reactome: R-HSA-9692916Reactome: R-HSA-9705671Reactome: R-HSA-9824878Reactome: R-HSA-9828211 |
| Cellular Localisation | CytoplasmEndosomeMitochondrionUndergoes Endocytosis Together With Tlr4 Upon Lps SignalingCo-Localized To Mitochondria With Trim35 |
| Alternative Antibody Names | Anti-Tnf Receptor-Associated Factor 3 antibodyAnti-Cd40 Receptor-Associated Factor 1 antibodyAnti-Craf1 antibodyAnti-Cd40-Binding Protein antibodyAnti-Cd40bp antibodyAnti-Lmp1-Associated Protein 1 antibodyAnti-Lap1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Traf3 antibodyAnti-TRAF3 antibodyAnti-CAP-1 antibodyAnti-CRAF1 antibodyAnti-TRAFAMN antibody |
Information sourced from Uniprot.org