Anti-TBP antibody (151-200 aa) (STJ95980)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TBP at the amino acid range 151-200
General Information
| Short Description | Rabbit polyclonal anti-TATA-box-binding protein (151-200 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TBP |
| Gene ID | 6908 |
| Uniprot ID | TBP_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TBP at the amino acid range 151-200 |
| Immunogen Region | 151-200 aa |
| Specificity | TFIID Polyclonal Antibody detects endogenous levels of TFIID protein. |
Additional Info
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5. TBP is a general transcription factor that functions at the core of the TFIID complex. During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. |
| Protein Name | Tata-Box-Binding ProteinTata Sequence-Binding ProteinTata-Binding FactorTata-Box FactorTranscription Initiation Factor Tfiid Tbp Subunit |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-427359Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-674695Reactome: R-HSA-6804756Reactome: R-HSA-6807505Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-749476Reactome: R-HSA-75953Reactome: R-HSA-76042Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071Reactome: R-HSA-9018519 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Tata-Box-Binding Protein antibodyAnti-Tata Sequence-Binding Protein antibodyAnti-Tata-Binding Factor antibodyAnti-Tata-Box Factor antibodyAnti-Transcription Initiation Factor Tfiid Tbp Subunit antibodyAnti-TBP antibodyAnti-GTF2D1 antibodyAnti-TF2D antibodyAnti-TFIID antibody |
Information sourced from Uniprot.org