Primary Antibodies | St John's Laboratory

Western blot analysis of mouse heart, using STX17 antibody (STJ11102869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-STX17 antibody (1-130) (STJ11102869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of mouse liver using UGT1A6 antibody (STJ11102868) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-UGT1A6 antibody (65-270) (STJ11102868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of CYB5R1 in paraffin-embedded Rat ovary using CYB5R1 Rabbit polyclonal antibody (STJ11102866) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CYB5R1 antibody (1-305) (STJ11102866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using HIBCH antibody (STJ11102854) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HIBCH antibody (33-330) (STJ11102854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SIRT3 Rabbit polyclonal antibody (STJ11102849) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT3 antibody (299-399) (STJ11102849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using ACOX3 Rabbit polyclonal antibody (STJ11102831) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ACOX3 antibody (10-100) (STJ11102831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of NFAT2 in paraffin-embedded human appendix using NFAT2 Rabbit polyclonal antibody (STJ11102802) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NFAT2 antibody (115-298) (STJ11102802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using Acetyl-HMGB1-K29 Rabbit polyclonal antibody (STJ11102770) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Acetyl-HMGB1-K29 antibody (STJ11102770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of von Willebrand factor (VWF) in paraffin-embedded human esophagus using von Willebrand factor (VWF) Rabbit polyclonal antibody (STJ11102753) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-VWF antibody (2645-2813) (STJ11102753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (STJ11102750) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Cyclin D1 antibody (200-295) (STJ11102750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ73761 (0. 5µg/ml) staining of Human Kidney lysate (A) + Peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ACE2 antibody (C-Term) (STJ73761)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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STJ73747 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Cathepsin B antibody (Internal) (STJ73747)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ73741 (0. 3µg/ml) staining of MCF7 nuclear (A) and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-SOX2 antibody (N-Term) (STJ73741)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/FC/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ73737 (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Estrogen receptor beta antibody (417-428) (STJ73737)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human

£312.50

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STJ73735 (6µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-ADRB1 antibody (Internal) (STJ73735)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Anti-mRaspberry antibody (STJ140289)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Anti-mPlum antibody (STJ140288)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Anti-CFP antibody (STJ140287)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Anti-GFP antibody (STJ140285)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£101.50 - £394.50

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Anti-mTopaz antibody (STJ140284)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Anti-mClover antibody (STJ140283)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Anti-mCerulean antibody (STJ140282)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Anti-mGrape antibody (STJ140281)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Anti-Azurite antibody (STJ140280)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Anti-mBanana antibody (STJ140279)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Immunofluorescence – anti-tdTomato antibody using hCEC cells transduced with tdTomato-CAV1; cells were fixed with methanol and anti-tdTomato at 1/250

Anti-tdTomato antibody (STJ140278)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

tdTomato/mCherry/RFP

£101.50 - £394.50

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Anti-GFP antibody {DyLight®633} (STJ140277)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

GFP

£141.50 - £426.50

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Anti-GFP antibody {DyLight®488} (STJ140275)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

GFP

£141.50 - £426.50

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Anti-Aquamarine antibody (STJ140271)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Anti-mApple antibody (STJ140269)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Anti-mBlueberry antibody (STJ140268)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Immunofluorescence –anti-pHluorin antibody using hCEC cells transduced with GFP-CAV1; cells were fixed with methanol and anti-pHluorin at 1/250

Anti-pHluorin antibody (STJ140262)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Immunofluorescence –anti-mNectarine antibody using hCEC cells transduced with mNectarine-Rab5a; cells were fixed with methanol and anti-mNectarine at 1/250

Anti-mNectarine antibody (STJ140261)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Immunofluorescence –anti-mAmetrine antibody usingNIH3T3 cells transduced with mAmetrine-Rab5a; cells were fixed with methanol and anti-mAmetrine at 1/250

Anti-mAmetrine antibody (STJ140259)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Immunofluorescence –anti-pHuji antibody using COS-7 cells transduced with pHuji-Rab5a; cells were fixed with methanol and anti-pHuji at 1/250

Anti-pHuji antibody (STJ140254)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Anti-BFP antibody at 1/2, 500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-BFP antibody (STJ140252)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Immunofluorescence – anti-mTurquoise antibody using NIH3T3 cells transduced with mTurquoise-Rab5a; cells were fixed with methanol and anti-mTurquoise at 1:100

Anti-mTurquoise antibody (STJ140247)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Immunofluorescence – anti-mOrange antibody using hCEC cells transduced with mOrange-Rab5a; cells were fixed with methanol and anti-mOrange at 1/250

Anti-mOrange antibody (STJ140227)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Immunofluorescence – anti-Sapphire antibody using NIH3T3 cells transduced with Sapphire-Rab5a; cells were fixed with methanol and anti-Sapphire at 1:100

Anti-Sapphire antibody (STJ140225)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£135.50 - £394.50

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Immunofluorescence – anti-mTangerine antibody using hCEC cells transduced with mTangerine-Rab5a; cells were fixed with methanol and anti-mTangerine at 1/250

Anti-mTangerine antibody (STJ140214)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

£135.50 - £394.50

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Immunofluorescence – anti-Miranda antibody in fly cells at 1:100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA

Anti-Miranda antibody (220 aa N-Term) (STJ140173)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

D.melanogaster

£135.50 - £394.50

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Anti-MANF antibody (STJA0002863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/IF/IHC

Reactivity :

Human

£438.50 - £2,502.50

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STJ73656 (2µg/ml) staining of K562 nuclear cell lysate (A) + peptide (B) and negative control Human Hippocampus (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-GATA1 antibody (C-Term) (STJ73656)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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STJ73653 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ATF6 antibody (C or N-Term) (STJ73653)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Sun, Mingjuan, et al. "Hedgehog pathway is involved in nitidine chloride induced inhibition of epithelial-mesenchymal transition and cancer stem cells-like properties in breast cancer cells. " Cell & bioscience 6. 1 (2016) : 44.

Anti-CD44 antibody (1105-1165 aa) (STJ193181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PTP4A1 antibody (STJ192209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of varius cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-POR antibody (STJ191940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Dietary fish oil improves autistic behaviors and gut homeostasis by altering the gut microbial composition in a mouse model of fragile X syndrome BRAIN BEHAVIOR AND IMMUNITY Yue Li WB mouse colon

Anti-TJP3 antibody (STJ191515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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