Anti-WAS antibody (256-305 aa) (STJ96261)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human WASP at the amino acid range 256-305
General Information
| Short Description | Rabbit polyclonal anti-Actin nucleation-promoting factor WAS (256-305 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WAS |
| Gene ID | 7454 |
| Uniprot ID | WASP_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human WASP at the amino acid range 256-305 |
| Immunogen Region | 256-305 aa |
| Specificity | WASP Polyclonal Antibody detects endogenous levels of WASP protein. |
Additional Info
| Post Translational Modifications | Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation. |
| Function | Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). |
| Protein Name | Actin Nucleation-Promoting Factor WasWiskott-Aldrich Syndrome ProteinWasp |
| Database Links | Reactome: R-HSA-202433Reactome: R-HSA-2029482Reactome: R-HSA-5663213Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013409Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonNucleus |
| Alternative Antibody Names | Anti-Actin Nucleation-Promoting Factor Was antibodyAnti-Wiskott-Aldrich Syndrome Protein antibodyAnti-Wasp antibodyAnti-WAS antibodyAnti-IMD2 antibody |
Information sourced from Uniprot.org