Anti-XPA antibody (211-260 aa) (STJ96279)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human XPA at the amino acid range 211-260
General Information
| Short Description | Rabbit polyclonal anti-DNA repair protein complementing XP-A cells (211-260 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | XPA |
| Gene ID | 7507 |
| Uniprot ID | XPA_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human XPA at the amino acid range 211-260 |
| Immunogen Region | 211-260 aa |
| Specificity | XPA Polyclonal Antibody detects endogenous levels of XPA protein. |
Additional Info
| Post Translational Modifications | ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage. Ubiquitinated by HERC2 leading to degradation by the proteasome. |
| Function | Involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3. Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH. |
| Protein Name | Dna Repair Protein Complementing Xp-A CellsXeroderma Pigmentosum Group A-Complementing Protein |
| Database Links | Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6781823Reactome: R-HSA-6782135 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Repair Protein Complementing Xp-A Cells antibodyAnti-Xeroderma Pigmentosum Group A-Complementing Protein antibodyAnti-XPA antibodyAnti-XPAC antibody |
Information sourced from Uniprot.org