• Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of VAMP-1/2/3 antibody in paraffin-embedded human lung carcinoma tissue.
  • Western blot analysis of mouse-brain cells using VAMP-1/2/3 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-VAMP-1/2/3 antibody (21-70 aa) (STJ96215)

SKU:
STJ96215

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vesicle-associated membrane protein 1 and Vesicle-associated membrane protein 2 and Vesicle-associated membrane protein 3 (21-70 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELI
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP2
VAMP3
6844
9341
6843
VAMP2_HUMAN
VAMP3_HUMAN
VAMP1_HUMAN
Immunogen Region: 21-70 aa
Specificity: VAMP-1/2/3 Polyclonal Antibody detects endogenous levels of VAMP-1/2/3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human VAMP-1/2/3 at the amino acid range 21-70
Post Translational Modifications (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release. BoNT/D has low catalytic activity on this protein due to its sequence. Note that humans are not known to be infected by C.botulinum type D. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets.
Function Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Protein Name Vesicle-Associated Membrane Protein 1
Vamp-1
Synaptobrevin-1
Database Links Reactome: R-HSA-5250955
Reactome: R-HSA-5250981
Reactome: R-HSA-5250989
Cellular Localisation Isoform 1: Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Single-Pass Type Iv Membrane Protein
Cell Junction
Synapse
Synaptosome
Isoform 2: Cytoplasmic Vesicle Membrane
Isoform 3: Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 1 antibody
Anti-Vamp-1 antibody
Anti-Synaptobrevin-1 antibody
Anti-VAMP1 antibody
Anti-SYB1 antibody

Information sourced from Uniprot.org

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