Anti-ZFYVE19 antibody (311-360 aa) (STJ96307)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ZFYVE19 at the amino acid range 311-360
STJ96307
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General Information

Short DescriptionRabbit polyclonal anti-Abscission/NoCut checkpoint regulator (311-360 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolZFYVE19
Gene ID84936
Uniprot IDANCHR_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ZFYVE19 at the amino acid range 311-360
Immunogen Region311-360 aa
SpecificityZFYVE19 Polyclonal Antibody detects endogenous levels of ZFYVE19 protein.

Additional Info

Post Translational Modifications Phosphorylated in vitro at Ser-22 by AURKB.however, phosphorylation at this site could not be confirmed in vivo.
Function Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.
Protein Name Abscission/Nocut Checkpoint Regulator
Anchr
Mll Partner Containing Fyve Domain
Zinc Finger Fyve Domain-Containing Protein 19
Database Links
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cleavage Furrow
Midbody
Midbody Ring
Localizes Mainly On Centrosomes In Interphase And Early Mitosis
Localizes At The Cleavage Furrow And Midbody Ring In Late Mitosis And Cytokinesis
Alternative Antibody Names Anti-Abscission/Nocut Checkpoint Regulator antibody
Anti-Anchr antibody
Anti-Mll Partner Containing Fyve Domain antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 19 antibody
Anti-ZFYVE19 antibody
Anti-ANCHR antibody
Anti-MPFYVE antibody

Information sourced from Uniprot.org

Citations

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