St John's Laboratory Ltd

Western blot analysis of extracts of various cell lines, using OTUB1 antibody (STJ112351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-OTUB1 antibody (50-150) (STJ112351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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ICC IF of human OTUB1 in HeLa cells with DAPI and Alexa Fluor 488 staining

Anti-Otubain-1/OTUB1 antibody (1-271aa) [AT17E10] (STJA0041755)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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SDS-PAGE gel of recombinant human Otubain-1 OTUB1 protein 3µg under reducing conditions Coomassie stain

Human Otubain-1/OTUB1 protein (Recombinant) (His-Tag) (STJP016497)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CNOT4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CNOT4 antibody (61-110 aa) (STJ92366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Rad18 Rabbit monoclonal antibody (STJ11102044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RAD18 antibody (1-100) [S4MR] (STJ11102044)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-UBR1 antibody (1260-1500) {FITC} (STJ503454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-MARCH2/MARCH-II antibody (Internal) (STJ71361)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human

£193.50

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Anti-RBCK1 antibody (STJA0007228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Human RBCK1 protein (Recombinant) (N-His) (STJP007924)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-UBR1 antibody (1260-1500) {Biotin} (STJ503453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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UBR1 Positive Control for STJ503454 peptide (STJ505243)

Applications :

WB

£308.50

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SDS-PAGE gel of recombinant human Otubain-2 OTUB2 protein under reducing conditions stained with Coomassie blue

Human Otubain-2/OTUB2 protein (Recombinant) (His-Tag) (STJP017174)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Western blot analysis of various lysates using RNF181 Rabbit polyclonal antibody (STJ116616) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF181 antibody (1-153) (STJ116616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot of human OTUB2 protein in 293T cells using anti-OTUB2 antibody

Anti-Otubain-2/OTUB2 antibody (1-234aa) [AT1F8] (STJA0041884)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of various lysates using RNF180 Rabbit polyclonal antibody (STJ117435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RNF180 antibody (1-416) (STJ117435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Cullin 2 antibody western blot K562 C6 3T3 HeLa STJA0034208

Anti-Cullin 2 antibody [R09-8A9] (STJA0034208)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of L929 cells using OTUB1 Rabbit polyclonal antibody (STJ113258) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-OTUB1 antibody (1-220) (STJ113258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Rat heart, using CUL5 Rabbit polyclonal antibody (STJ27322) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CUL5 antibody (1-300) (STJ27322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70352 (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

Anti-EDD1/HYD antibody (C-Term) (STJ70352)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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Rat SOCS2 (Suppressors Of Cytokine Signaling 2) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018648)

Applications :

ELISA

Reactivity :

Rat

£473.50 - £659.50

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Western blot analysis of lysates from Rat liver, using TRIM65 Rabbit pAb (STJ11106123) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-TRIM65 antibody (380-490 aa) (STJ11106123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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TRIF Positive Control for STJ503401 peptide (STJ505231)

Applications :

WB

£308.50

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Human SKP1 (S-Phase Kinase Associated Protein 1) Sandwich ELISA Kit (STJE0012182)

Applications :

ELISA

Reactivity :

Human

£421.50 - £586.50

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Anti-Recombinant-SKP1 antibody [RM2A68] (STJA0025042)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-OTUB1 antibody [1000] (STJA0032690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat

£224.50 - £308.50

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Immunohistochemistry of rat tissue with Anti-TRIF primary antibody (STJ503399) at 1:250 dilution in Buffer. 1) TRIF Positive Control peptide 2) Rat adipose Tissue.

Anti-TRIF antibody (STJ503399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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SDS-PAGE gel of recombinant human UBL3 protein 3μg under reducing conditions with Coomassie blue stain

Human UBL3 protein (Recombinant) (His-Tag) (STJP017216)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Anti-CUL5 antibody (560-640 aa) (STJ191691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using RNF126 rabbit polyclonal antibody (STJ11101788) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RNF126 antibody (200-300) (STJ11101788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using UBE4B Rabbit polyclonal antibody (STJ119673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE4B antibody (130-250) (STJ119673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-SYVN1 antibody (460-510 aa) (STJ13102272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Human/Rat

£322.50

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Immunofluorescence analysis of L929 cells using SIAH1 Rabbit polyclonal antibody (STJ25527) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SIAH1 antibody (1-313) (STJ25527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of L-929 cells using USP46 Rabbit polyclonal antibody (STJ119875) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-USP46 antibody (100-200) (STJ119875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TRIF antibody {FITC} (STJ503401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Rat SOCS2 (Suppressors Of Cytokine Signaling 2) Sandwich ELISA Kit (STJE0012261)

Applications :

ELISA

Reactivity :

Rat

£451.50 - £629.50

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SDS-PAGE gel of recombinant human SKP1 protein 3µg under reducing conditions with Coomassie blue stain

Human SKP1 protein (Recombinant) (No-Tag) (STJP016572)

Host :

E.coli

Applications :

SDS-PAGE

£125.50 - £649.50

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Western blot analysis of various lysates using RBX1 Rabbit polyclonal antibody (STJ116711) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RBX1 antibody (1-100) (STJ116711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Anti-RBX1 antibody (STJA0038046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£187.50 - £281.50

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Western blot analysis of various lysates using OTUB2 Rabbit polyclonal antibody (STJ118690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-OTUB2 antibody (1-73) (STJ118690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-Cullin5 antibody (N-Term) (STJ13100099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£322.50

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Anti-Recombinant-CUL5 antibody [RM2S34] (STJA0026592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g USP14 antibody (STJ11103046). Western blot was performed from the immunoprecipitate using USP14 antibody (STJ11103046) at a dilution of 1:2000.

Anti-USP14 antibody (371-473) [S6MR] (STJ11103046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-TRIF antibody {Biotin} (STJ503400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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TRIF Blocking Peptide peptide (STJ505036)

Applications :

Immunodepletion/Immunocompetition

£260.50

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STJ70109 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SIAH1 antibody (N-Term) (STJ70109)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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SDS-PAGE gel of recombinant human SIAH1 protein 3µg under reducing conditions Coomassie blue stain

Human SIAH1 protein (Recombinant) (His-Tag) (STJP017778)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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RBX1 antibody western blot Jurkat THP-1 C6 3T3 HeLa STJA0034819

Anti-RBX1 antibody [R07-3F6] (STJA0034819)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Biotinylated STJ70109 (2µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-SIAH1, Biotinylated antibody (N-Term) {Biotin} (STJ73231)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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