St John's Laboratory Ltd

Western blot of human OTUB2 protein in 293T cells using anti-OTUB2 antibody

Anti-Otubain-2/OTUB2 antibody (1-234aa) [AT1F8] (STJA0041884)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

$302.91 - $458.85

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Western blot analysis of extracts of various cell lines, using OTUB1 antibody (STJ112351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-OTUB1 antibody (50-150) (STJ112351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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ICC IF of human OTUB1 in HeLa cells with DAPI and Alexa Fluor 488 staining

Anti-Otubain-1/OTUB1 antibody (1-271aa) [AT17E10] (STJA0041755)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$302.91 - $458.85

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SDS-PAGE gel of recombinant human Otubain-1 OTUB1 protein 3µg under reducing conditions Coomassie stain

Human Otubain-1/OTUB1 protein (Recombinant) (His-Tag) (STJP016497)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Immunofluorescence analysis of L929 cells using OTUB1 Rabbit polyclonal antibody (STJ113258) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-OTUB1 antibody (1-220) (STJ113258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from Rat heart, using CUL5 Rabbit polyclonal antibody (STJ27322) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CUL5 antibody (1-300) (STJ27322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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STJ70352 (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

Anti-EDD1/HYD antibody (C-Term) (STJ70352)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

$431.25

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Western blot analysis of extracts of MCF7 cells, using Rad18 Rabbit polyclonal antibody (STJ27333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-RAD18 antibody (216-495) (STJ27333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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UBR1 Blocking Peptide for STJ503454 peptide (STJ505050)

Applications :

Immunodepletion/Immunocompetition

$359.49

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SDS-PAGE gel of recombinant human USP14 protein 3µg stained with Coomassie blue under reducing conditions

Human USP14 protein (Recombinant) (His-Tag) (STJP018028)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Cullin 2 antibody western blot K562 C6 3T3 HeLa STJA0034208

Anti-Cullin 2 antibody [R09-8A9] (STJA0034208)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-CUL5 antibody (560-640 aa) (STJ191691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using Rad18 Rabbit monoclonal antibody (STJ11102044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RAD18 antibody (1-100) [S4MR] (STJ11102044)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-UBR1 antibody (1260-1500) {FITC} (STJ503454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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SDS-PAGE gel of recombinant human Otubain-2 OTUB2 protein under reducing conditions stained with Coomassie blue

Human Otubain-2/OTUB2 protein (Recombinant) (His-Tag) (STJP017174)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Cullin 2 antibody western blot Raji lysates STJA0036904

Anti-Cullin 2 antibody [R06-4T1] (STJA0036904)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Rat SOCS2 (Suppressors Of Cytokine Signaling 2) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018648)

Applications :

ELISA

Reactivity :

Rat

$653.43 - $910.11

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Western blot analysis of lysates from Rat liver, using TRIM65 Rabbit pAb (STJ11106123) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-TRIM65 antibody (380-490 aa) (STJ11106123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using UBA1 Rabbit mAb (STJ11102462) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-UBA1 antibody (959-1058) [S2MR] (STJ11102462)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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STJ71616 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-OTUB2 antibody (Internal) (STJ71616)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow

$431.25

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Anti-USP31 antibody (Internal) (STJ73818)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

$431.25

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Immunofluorescence analysis of L929 cells using SIAH1 Rabbit polyclonal antibody (STJ25527) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SIAH1 antibody (1-313) (STJ25527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates, using RNF126 rabbit polyclonal antibody (STJ11101788) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RNF126 antibody (200-300) (STJ11101788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using UBE4B Rabbit polyclonal antibody (STJ119673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE4B antibody (130-250) (STJ119673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-SYVN1 antibody (460-510 aa) (STJ13102272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Human/Rat

$445.05

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Anti-OTUB1 antibody [1000] (STJA0032690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat

$309.81 - $425.73

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Western blot analysis of various lysates using RBX1 Rabbit polyclonal antibody (STJ116711) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RBX1 antibody (1-100) (STJ116711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Anti-RBX1 antibody (STJA0038046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$258.75 - $388.47

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Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CNOT4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CNOT4 antibody (61-110 aa) (STJ92366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates using RNF181 Rabbit polyclonal antibody (STJ116616) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF181 antibody (1-153) (STJ116616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using RNF180 Rabbit polyclonal antibody (STJ117435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RNF180 antibody (1-416) (STJ117435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-MonoMethyl-Histone H3-Lys23 antibody [2Q7-D8-T7] (STJA0033688)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$309.81 - $440.91

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RBX1 antibody western blot Jurkat THP-1 C6 3T3 HeLa STJA0034819

Anti-RBX1 antibody [R07-3F6] (STJA0034819)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunofluorescence analysis of L-929 cells using USP46 Rabbit polyclonal antibody (STJ119875) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-USP46 antibody (100-200) (STJ119875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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SDS-PAGE gel of recombinant human SKP1 protein 3µg under reducing conditions with Coomassie blue stain

Human SKP1 protein (Recombinant) (No-Tag) (STJP016572)

Host :

E.coli

Applications :

SDS-PAGE

$173.19 - $896.31

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Western blot analysis of various lysates using SKP1 Rabbit mAb (STJ11102409) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SKP1 antibody (64-163) [S9MR] (STJ11102409)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of extracts of various cell lines, using SKP1 antibody (STJ25541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SKP1 antibody (1-163) (STJ25541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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SKP1 antibody western blot K562 rat brain C6 3T3 STJA0034861

Anti-SKP1 antibody [R01-6H2] (STJA0034861)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of various lysates using OTUB2 Rabbit polyclonal antibody (STJ118690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-OTUB2 antibody (1-73) (STJ118690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunofluorescence analysis of NIH-3T3 cells using TRIM31 Rabbit polyclonal antibody (STJ11101162) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TRIM31 antibody (186-425) (STJ11101162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded mouse heart using NHLRC1 antibody (STJ28752) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NHLRC1 antibody (1-280) (STJ28752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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UFD1L antibody western blot HeLa lysates STJA0034979

Anti-UFD1 antibody [R09-7G5] (STJA0034979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$184.23 - $388.47

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Rat SOCS2 (Suppressors Of Cytokine Signaling 2) Sandwich ELISA Kit (STJE0012261)

Applications :

ELISA

Reactivity :

Rat

$623.07 - $868.71

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Western blot analysis of various cells using Skp1 p19 Polyclonal Antibody

Anti-SKP1 antibody (41-90 aa) (STJ95675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using CBLC antibody (STJ110099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CBLC antibody (245-474) (STJ110099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunofluorescence analysis of U-2 OS cells using UBE2C Rabbit monoclonal antibody (STJ11102292) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-UBE2C antibody (70-150) [S2MR] (STJ11102292)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.57 - $765.21

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SDS-PAGE gel of recombinant human UbcH10 UBE2C protein under reducing conditions stained with Coomassie blue

Human UbcH10/UBE2C protein (Recombinant) (His-Tag) (STJP017790)

Host :

E.coli

Applications :

SDS-PAGE

$185.61 - $1,019.13

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Anti-USP5 antibody (STJA0033167)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$258.75 - $388.47

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