| Short Description : | Rabbit polyclonal TRIM31 (186-425) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRIM31 |
| Gene ID: | 11074 |
| Uniprot ID: | TRI31_HUMAN |
| Immunogen Region: | 186-425 |
| Immunogen Sequence: | ELLHQVLEEEKNFLLSRIYW LGHEGTEAGKHYVASTEPQL NDLKKLVDSLKTKQNMPPRQ LLEDIKVVLCRSEEFQFLNP TPVPLELEKKLSEAKSRHDS ITGSLKKFKDQLQADRKKDE NRFFKSMNKNDMKSWGLLQK NNHKMNKTSEPGSSSAGGRT TSGPPNHHSSAPSHSLFRAS SAGKVTFPVCLLASYDEISG QGASSQDTKTFDVALSEELH AALSEWLTAIRAWFCEVPS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-425 of human TRIM31 (NP_008959.3). |
| Tissue Specificity | Up-regulated in gastric adenocarcinomas. |
| Post Translational Modifications | Auto-ubiquitinated (in vitro). |
| Function | E3 ubiquitin-protein ligase that acts as a regulator of antiviral immune response and inflammation by mediating ubiquitination of substrates. Acts as a regulator of innate immune defense against viruses by mediating 'Lys-63'-linked ubiquitination of MAVS, promoting MAVS polymerization and formation of three-stranded helical filaments on mitochondria. Acts as a negative regulator of the NLRP3 inflammasome by catalyzing 'Lys-48'-linked ubiquitination of NLRP3, leading to its degradation. Regulator of Src-induced anchorage independent cell growth. |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim31Tripartite Motif-Containing Protein 31 |
| Database Links | Reactome: R-HSA-877300 |
| Cellular Localisation | CytoplasmMitochondrionPredominantly Expressed In The Cytoplasm But A Fraction Is Associated With The MitochondriaRecruited T Omitochondria After Viral Infection |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim31 antibodyAnti-Tripartite Motif-Containing Protein 31 antibodyAnti-TRIM31 antibodyAnti-C6orf13 antibodyAnti-RNF antibody |
Information sourced from Uniprot.org
