• Immunofluorescence analysis of NIH-3T3 cells using TRIM31 Rabbit polyclonal antibody (STJ11101162) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TRIM31 Rabbit polyclonal antibody (STJ11101162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TRIM31 antibody (186-425) (STJ11101162)

SKU:
STJ11101162

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM31 (186-425) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM31
Gene ID: 11074
Uniprot ID: TRI31_HUMAN
Immunogen Region: 186-425
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-425 of human TRIM31 (NP_008959.3).
Immunogen Sequence: ELLHQVLEEEKNFLLSRIYW LGHEGTEAGKHYVASTEPQL NDLKKLVDSLKTKQNMPPRQ LLEDIKVVLCRSEEFQFLNP TPVPLELEKKLSEAKSRHDS ITGSLKKFKDQLQADRKKDE NRFFKSMNKNDMKSWGLLQK NNHKMNKTSEPGSSSAGGRT TSGPPNHHSSAPSHSLFRAS SAGKVTFPVCLLASYDEISG QGASSQDTKTFDVALSEELH AALSEWLTAIRAWFCEVPS
Tissue Specificity Up-regulated in gastric adenocarcinomas.
Post Translational Modifications Auto-ubiquitinated (in vitro).
Function E3 ubiquitin-protein ligase that acts as a regulator of antiviral immune response and inflammation by mediating ubiquitination of substrates. Acts as a regulator of innate immune defense against viruses by mediating 'Lys-63'-linked ubiquitination of MAVS, promoting MAVS polymerization and formation of three-stranded helical filaments on mitochondria. Acts as a negative regulator of the NLRP3 inflammasome by catalyzing 'Lys-48'-linked ubiquitination of NLRP3, leading to its degradation. Regulator of Src-induced anchorage independent cell growth.
Protein Name E3 Ubiquitin-Protein Ligase Trim31
Tripartite Motif-Containing Protein 31
Database Links Reactome: R-HSA-877300
Cellular Localisation Cytoplasm
Mitochondrion
Predominantly Expressed In The Cytoplasm But A Fraction Is Associated With The Mitochondria
Recruited T Omitochondria After Viral Infection
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim31 antibody
Anti-Tripartite Motif-Containing Protein 31 antibody
Anti-TRIM31 antibody
Anti-C6orf13 antibody
Anti-RNF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance