Western blot analysis of various lysates using UBA1 Rabbit mAb (STJ11102462) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-UBA1 antibody (959-1058) [S2MR] (STJ11102462)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102462
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General Information

Short DescriptionRabbit monoclonal UBA1 (959-1058) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS2MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:6000
IHC-P:1:100-1:1000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolUBA1
Gene ID7317
Uniprot IDUBA1_HUMAN
Immunogen Region959-1058
Immunogen SequenceEVQGLQPNGEEMTLKQFLDY FKTEHKLEITMLSQGVSMLY SFFMPAAKLKERLDQPMTEI VSRVSKRKLGRHVRALVLEL CCNDESGEDVEVPYVRYTIR
SpecificityA synthetic peptide corresponding to a sequence within amino acids 959-1058 of human UBA1 (P22314).

Additional Info

Tissue Specificity Detected in erythrocytes (at protein level). Ubiquitous.
Post Translational Modifications ISGylated.
Function Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
Protein Name Ubiquitin-Like Modifier-Activating Enzyme 1
Protein A1s9
Ubiquitin-Activating Enzyme E1
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Mitochondrion
Nucleus
Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Ubiquitin-Like Modifier-Activating Enzyme 1 antibody
Anti-Protein A1s9 antibody
Anti-Ubiquitin-Activating Enzyme E1 antibody
Anti-UBA1 antibody
Anti-A1S9T antibody
Anti-UBE1 antibody

Information sourced from Uniprot.org

Citations

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