• Western blot analysis of various lysates using RBX1 Rabbit polyclonal antibody (STJ116711) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RBX1 antibody (1-100) (STJ116711)

SKU:
STJ116711

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBX1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBX1
Gene ID: 9978
Uniprot ID: RBX1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RBX1 (NP_055063.1).
Immunogen Sequence: MAAAMDVDTPSGTNSGAGKK RFEVKKWNAVALWAWDIVVD NCAICRNHIMDLCIECQANQ ASATSEECTVAWGVCNHAFH FHCISRWLKTRQVCPLDNRE
Tissue Specificity Widely expressed.
Function E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Recruits the E2 ubiquitin-conjugating enzyme CDC34 to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5.polyubiquitinates monoubiquitinated POLR2A.
Protein Name E3 Ubiquitin-Protein Ligase Rbx1
E3 Ubiquitin-Protein Transferase Rbx1
Protein Zyp
Ring Finger Protein 75
Ring-Box Protein 1
Rbx1
Regulator Of Cullins 1
Roc1 Cleaved Into - E3 Ubiquitin-Protein Ligase Rbx1 - N-Terminally Processed
E3 Ubiquitin-Protein Transferase Rbx1 - N-Terminally Processed
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-1170546
Reactome: R-HSA-1234176
Reactome: R-HSA-180585
Reactome: R-HSA-195253
Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2644607
Reactome: R-HSA-2894862
Reactome: R-HSA-4641258
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5632684
Reactome: R-HSA-5658442
Reactome: R-HSA-5696394
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-68949
Reactome: R-HSA-8854050
Reactome: R-HSA-8939902
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-9020702
Reactome: R-HSA-9604323
Reactome: R-HSA-9679191
Reactome: R-HSA-9708530
Reactome: R-HSA-9725371
Reactome: R-HSA-9755511
Reactome: R-HSA-9762114
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rbx1 antibody
Anti-E3 Ubiquitin-Protein Transferase Rbx1 antibody
Anti-Protein Zyp antibody
Anti-Ring Finger Protein 75 antibody
Anti-Ring-Box Protein 1 antibody
Anti-Rbx1 antibody
Anti-Regulator Of Cullins 1 antibody
Anti-Roc1 Cleaved Into - E3 Ubiquitin-Protein Ligase Rbx1 - N-Terminally Processed antibody
Anti-E3 Ubiquitin-Protein Transferase Rbx1 - N-Terminally Processed antibody
Anti-RBX1 antibody
Anti-RNF75 antibody
Anti-ROC1 antibody

Information sourced from Uniprot.org

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