Anti-NHLRC1 antibody (1-280) (STJ28752)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NHLRC1 (1-280) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:2000-1:7000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NHLRC1 |
| Gene ID | 378884 |
| Uniprot ID | NHLC1_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MAAEASESGPALHELMREAE ISLLECKVCFEKFGHRQQRR PRNLSCGHVVCLACVAALAH PRTLALECPFCRRACRGCDT SDCLPVLHLIELLGSALRQS PAAHRAAPSAPGALTCHHTF GGWGTLVNPTGLALCPKTGR VVVVHDGRRRVKIFDSGGGC AHQFGEKGDAAQDIRYPVDV TITNDCHVVVTDAGDRSIKV FDFFGQIKLVIGGQFSLPWG VETTPQNGIVVTDAEAGSL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NHLRC1 (NP_940988.2). |
Additional Info
| Tissue Specificity | Expressed in brain, cerebellum, spinal cord, medulla, heart, liver, skeletal muscle and pancreas. |
| Function | E3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. |
| Protein Name | E3 Ubiquitin-Protein Ligase Nhlrc1MalinNhl Repeat-Containing Protein 1Ring-Type E3 Ubiquitin Transferase Nhlrc1 |
| Database Links | Reactome: R-HSA-3322077Reactome: R-HSA-3785653 |
| Cellular Localisation | Endoplasmic ReticulumNucleusLocalizes At The Endoplasmic Reticulum AndTo A Lesser ExtentIn The Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Nhlrc1 antibodyAnti-Malin antibodyAnti-Nhl Repeat-Containing Protein 1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Nhlrc1 antibodyAnti-NHLRC1 antibodyAnti-EPM2B antibody |
Information sourced from Uniprot.org