• Immunohistochemistry analysis of paraffin-embedded mouse heart using NHLRC1 antibody (STJ28752) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using NHLRC1 antibody (STJ28752) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of Rat heart, using NHLRC1 Rabbit polyclonal antibody (STJ28752) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NHLRC1 antibody (1-280) (STJ28752)

SKU:
STJ28752

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NHLRC1 (1-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:7000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NHLRC1
Gene ID: 378884
Uniprot ID: NHLC1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NHLRC1 (NP_940988.2).
Immunogen Sequence: MAAEASESGPALHELMREAE ISLLECKVCFEKFGHRQQRR PRNLSCGHVVCLACVAALAH PRTLALECPFCRRACRGCDT SDCLPVLHLIELLGSALRQS PAAHRAAPSAPGALTCHHTF GGWGTLVNPTGLALCPKTGR VVVVHDGRRRVKIFDSGGGC AHQFGEKGDAAQDIRYPVDV TITNDCHVVVTDAGDRSIKV FDFFGQIKLVIGGQFSLPWG VETTPQNGIVVTDAEAGSL
Tissue Specificity Expressed in brain, cerebellum, spinal cord, medulla, heart, liver, skeletal muscle and pancreas.
Function E3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway.
Protein Name E3 Ubiquitin-Protein Ligase Nhlrc1
Malin
Nhl Repeat-Containing Protein 1
Ring-Type E3 Ubiquitin Transferase Nhlrc1
Database Links Reactome: R-HSA-3322077
Reactome: R-HSA-3785653
Cellular Localisation Endoplasmic Reticulum
Nucleus
Localizes At The Endoplasmic Reticulum And
To A Lesser Extent
In The Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Nhlrc1 antibody
Anti-Malin antibody
Anti-Nhl Repeat-Containing Protein 1 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Nhlrc1 antibody
Anti-NHLRC1 antibody
Anti-EPM2B antibody

Information sourced from Uniprot.org

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