| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CUL5 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CUL5 |
| Gene ID: |
8065 |
| Uniprot ID: |
CUL5_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CUL5 (NP_003469.2). |
| Immunogen Sequence: |
MATSNLLKNKGSLQFEDKWD FMRPIVLKLLRQESVTKQQW FDLFSDVHAVCLWDDKGPAK IHQALKEDILEFIKQAQARV LSHQDDTALLKAYIVEWRKF FTQCDILPKPFCQLEITLMG KQGSNKKSNVEDSIVRKLML DTWNESIFSNIKNRLQDSAM KLVHAERLGEAFDSQLVIGV RESYVNLCSNPEDKLQIYRD NFEKAYLDSTERFYRTQAPS YLQQNGVQNYMKYADAKLK |
| Post Translational Modifications | Neddylated.which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN). |
| Function | Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine. As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5.polyubiquitinates monoubiquitinated POLR2A. May form a cell surface vasopressin receptor. (Microbial infection) Seems to be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. |
| Protein Name | Cullin-5Cul-5Vasopressin-Activated Calcium-Mobilizing Receptor 1Vacm-1 |
| Database Links | Reactome: R-HSA-180585Reactome: R-HSA-8863795Reactome: R-HSA-8951664Reactome: R-HSA-9705462Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusLocalizes To Sites Of Dna Damage In A Ubap2 And Ubap2l-Dependent Manner |
| Alternative Antibody Names | Anti-Cullin-5 antibodyAnti-Cul-5 antibodyAnti-Vasopressin-Activated Calcium-Mobilizing Receptor 1 antibodyAnti-Vacm-1 antibodyAnti-CUL5 antibodyAnti-VACM1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
