• Western blot analysis of various lysates using RNF180 Rabbit polyclonal antibody (STJ117435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RNF180 antibody (1-416) (STJ117435)

SKU:
STJ117435

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNF180 (1-416) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF180
Gene ID: 285671
Uniprot ID: RN180_HUMAN
Immunogen Region: 1-416
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-416 of human RNF180 (NP_848627.1).
Immunogen Sequence: MKRSKELITKNHSQEETSIL RCWKCRKCIASSGCFMEYLE NQVIKDKDDSVDAQNICHVW HMNVEALPEWISCLIQKAQW TVGKLNCPFCGARLGGFNFV STPKCSCGQLAAVHLSKSRT DYQPTQAGRLMRPSVKYLSH PRVQSGCDKEALLTGGGSEN RNHRLLNMARNNNDPGRLTE ALCLEVRPTYFEMKNEKLLS KASEPKYQLFVPQLVTGRCA TRAFHRKSHSLDLNISEKL
Function E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2.
Protein Name E3 Ubiquitin-Protein Ligase Rnf180
Ring Finger Protein 180
Ring-Type E3 Ubiquitin Transferase Rnf180
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Nucleus Envelope
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf180 antibody
Anti-Ring Finger Protein 180 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf180 antibody
Anti-RNF180 antibody

Information sourced from Uniprot.org

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