• Western blot analysis of extracts of various cell lines, using SKP1 antibody (STJ25541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SKP1 antibody (1-163) (STJ25541)

SKU:
STJ25541

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SKP1 (1-163) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SKP1
Gene ID: 6500
Uniprot ID: SKP1_HUMAN
Immunogen Region: 1-163
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human SKP1 (NP_733779.1).
Immunogen Sequence: MPSIKLQSSDGEIFEVDVEI AKQSVTIKTMLEDLGMDDEG DDDPVPLPNVNAAILKKVIQ WCTHHKDDPPPPEDDENKEK RTDDIPVWDQEFLKVDQGTL FELILAANYLDIKGLLDVTC KTVANMIKGKTPEEIRKTFN IKNDFTEEEEAQVRKENQWC EEK
Post Translational Modifications Undergoes autophagy-mediated degradation in the liver in a time-dependent manner.
Function Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'.the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.
Protein Name S-Phase Kinase-Associated Protein 1
Cyclin-A/Cdk2-Associated Protein P19
P19a
Organ Of Corti Protein 2
Ocp-2
Organ Of Corti Protein Ii
Ocp-Ii
Rna Polymerase Ii Elongation Factor-Like Protein
Siii
Transcription Elongation Factor B Polypeptide 1-Like
P19skp1
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1170546
Reactome: R-HSA-174113
Reactome: R-HSA-180534
Reactome: R-HSA-187577
Reactome: R-HSA-195253
Reactome: R-HSA-202424
Reactome: R-HSA-2122947
Reactome: R-HSA-2565942
Reactome: R-HSA-2644606
Reactome: R-HSA-2644607
Reactome: R-HSA-2871837
Reactome: R-HSA-2894862
Reactome: R-HSA-400253
Reactome: R-HSA-5607761
Reactome: R-HSA-5607764
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5676590
Reactome: R-HSA-5684264
Reactome: R-HSA-68949
Reactome: R-HSA-69231
Reactome: R-HSA-8854050
Reactome: R-HSA-8939902
Reactome: R-HSA-8951664
Reactome: R-HSA-9020702
Reactome: R-HSA-917937
Reactome: R-HSA-9604323
Reactome: R-HSA-9708530
Reactome: R-HSA-9725371
Reactome: R-HSA-9762114
Reactome: R-HSA-983168
Alternative Antibody Names Anti-S-Phase Kinase-Associated Protein 1 antibody
Anti-Cyclin-A/Cdk2-Associated Protein P19 antibody
Anti-P19a antibody
Anti-Organ Of Corti Protein 2 antibody
Anti-Ocp-2 antibody
Anti-Organ Of Corti Protein Ii antibody
Anti-Ocp-Ii antibody
Anti-Rna Polymerase Ii Elongation Factor-Like Protein antibody
Anti-Siii antibody
Anti-Transcription Elongation Factor B Polypeptide 1-Like antibody
Anti-P19skp1 antibody
Anti-SKP1 antibody
Anti-EMC19 antibody
Anti-OCP2 antibody
Anti-SKP1A antibody
Anti-TCEB1L antibody

Information sourced from Uniprot.org

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