Anti-RAD18 antibody (216-495) (STJ27333)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Rad18 (216-495) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAD18 |
| Gene ID | 56852 |
| Uniprot ID | RAD18_HUMAN |
| Immunogen Region | 216-495 |
| Immunogen Sequence | INKHLDSCLSREEKKESLRS SVHKRKPLPKTVYNLLSDRD LKKKLKEHGLSIQGNKQQLI KRHQEFVHMYNAQCDALHPK SAAEIVREIENIEKTRMRLE ASKLNESVMVFTKDQTEKEI DEIHSKYRKKHKSEFQLLVD QARKGYKKIAGMSQKTVTIT KEDESTEKLSSVCMGQEDNM TSVTNHFSQSKLDSPEELEP DREEDSSSCIDIQEVLSSSE SDSCNSSSSDIIRDLLEEE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human Rad18 (NP_064550.3). |
Additional Info
| Function | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rad18Postreplication Repair Protein Rad18Hhr18Hrad18Ring Finger Protein 73Ring-Type E3 Ubiquitin Transferase Rad18 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-8866654 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeAssociates With ChromatinColocalizes With Slf1 In The Nucleus And To CentrosomesRelocalizes With Slf1 To Nuclear Foci In Response To Dna DamageAccumulates With The Slf1-Slf2 And Smc5-Smc6 Complexes At Replication-Coupled Dna Interstrand Repair And Dna Double-Strand Breaks (Dsbs) Sites On Chromatin In A Ubiquitin-Dependent Manner |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rad18 antibodyAnti-Postreplication Repair Protein Rad18 antibodyAnti-Hhr18 antibodyAnti-Hrad18 antibodyAnti-Ring Finger Protein 73 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rad18 antibodyAnti-RAD18 antibodyAnti-RNF73 antibody |
Information sourced from Uniprot.org