St John's Laboratory Ltd

Western blot analysis of various lysates using UBE2S Rabbit pAb (STJ26022) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-UBE2S antibody (1-222) (STJ26022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SDS-PAGE gel showing recombinant human E2-EPF UBE2S protein 3 μg under reducing conditions with Coomassie blue staining

Human E2-EPF/UBE2S protein (Recombinant) (His-Tag) (STJP016514)

Host :

E.coli

Applications :

SDS-PAGE

£110.50 - £535.50

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Western blot with Anti-ITCH Antibody (STJ501506) of THP cell at 1:500 dilution in buffer.

Anti-ITCH antibody (250-300) (STJ501506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Western blot analysis of various lysates using HERC3 Rabbit pAb (STJ111302) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HERC3 antibody (1-200) (STJ111302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using RNF166 antibody (STJ112846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNF166 antibody (1-237) (STJ112846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-NEDD4 antibody (300-350) (STJ506276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£400.50

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Anti-NEDD4 antibody (300-350) {FITC} (STJ506278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£497.50

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Anti-COP1/RFWD2 antibody (582-731 aa) [SRM5264] (STJ11107166)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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SDS-PAGE gel of recombinant human Ubc16 UBE2W protein 3µg Coomassie stain

Human Ubc16/UBE2W protein (Recombinant) (His-Tag) (STJP017214)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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SDS-PAGE gel of recombinant human UBE2T protein under reducing conditions stained with Coomassie blue

Human UBE2T protein (Recombinant) (His-Tag) (STJP016669)

Host :

E.coli

Applications :

SDS-PAGE

£125.50 - £649.50

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Anti-NEDD4 antibody (300-350) {Biotin} (STJ506277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£497.50

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Human OTUB1 protein (Recombinant) (N-GST) (STJP014683)

Host :

E.coli

Applications :

ELISA/WB

£319.50

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Western blot analysis of various cells using CUL-3 Polyclonal Antibody diluted at 1：1000

Anti-CUL3 antibody (1-50 aa) (STJ92524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Recombinant-CUL3 antibody [RM2E80] (STJA0025450)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Cullin 3 antibody western blot PC-12 rat spleen STJA0034209

Anti-Cullin 3 antibody [R09-4D1] (STJA0034209)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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SDS-PAGE gel showing recombinant human UBE2G1 protein 3 µg under reducing conditions with Coomassie blue stain

Human UBE2G1 protein (Recombinant) (His-Tag) (STJP018980)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Western blot analysis of various lysates using RMND5A Rabbit polyclonal antibody (STJ117123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RMND5A antibody (200-270) (STJ117123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using USP48 Rabbit polyclonal antibody (STJ27046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-USP48 antibody (1-300) (STJ27046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CUL4A antibody (590-670 aa) (STJ191690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunoprecipitation analysis of 300 Mu g extracts of Mouse lung using 3 Mu g SMURF2 antibody (STJ11101803). Western blot was performed from the immunoprecipitate using SMURF2 antibody (STJ11101803) at a dilution of 1:1000.

Anti-SMURF2 antibody (201-290) [S3MR] (STJ11101803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of various lysates using ARIH1 Rabbit polyclonal antibody (STJ11102851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARIH1 antibody (400 aa) (STJ11102851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot WWP1 expression in mouse liver and PC3 cells using antibody STJA0041456

Anti-WWP1 antibody (N-Term) (STJA0041456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat

£474.50

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Western blot analysis of various lysates using RNF144A Rabbit polyclonal antibody (STJ119664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RNF144A antibody (52-179) (STJ119664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of various cells using CUL-1 Polyclonal Antibody

Anti-CUL1 antibody (727-776 aa) (STJ92522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using LNX1 rabbit polyclonal antibody (STJ28564) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LNX1 antibody (501-728) (STJ28564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SMURF2 antibody western blot SH-SY5Y lysates STJA0036637

Anti-SMURF2 antibody [R06-5K4] (STJA0036637)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-NEDD4 antibody (STJ13101434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

£322.50

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Immunofluorescence analysis of PC-12 cells using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-USP9Y antibody (2456-2555) (STJ11103439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ73313 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Cullin 3 antibody (Internal) (STJ73313)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

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Western blot analysis of various lysates using NEDD4L Rabbit mAb (STJ11103647) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-NEDD4L antibody (876-975) [S7MR] (STJ11103647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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STJ71108 (0. 1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ITCH/AIF4 antibody (Internal) (STJ71108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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ITCH/AIP4 Blocking Peptide peptide (STJ504270)

Applications :

Immunodepletion/Immunocompetition

£260.50

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ITCH/AIP4 antibody western blot A549 HL-60 STJA0035242

Anti-ITCH antibody [R03-4I6] (STJA0035242)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using UBE2T antibody (STJ28935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2T antibody (1-197) (STJ28935)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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SFT antibody western blot Raw2647 STJA0012589

Anti-UBE2D1 antibody (119-132 aa) [R07-4E8] (STJA0012589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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STJ71436 (0. 2µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TEB4/MARCH-VI antibody (701-712) (STJ71436)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse

£312.50

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USP11 antibody western blot Jurkat rat brain HeLa STJA0033889

Anti-USP11 antibody [R08-2D7] (STJA0033889)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of various lysates, using UBE3A antibody (STJ29866) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-UBE3A antibody (300-590) (STJ29866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using RNF31 Rabbit pAb (STJ110526) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RNF31 antibody (500-700) (STJ110526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-Recombinant-CUL1 antibody [RM2E77] (STJA0025447)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Immunohistochemistry analysis of paraffin-embedded rat brain using UBE4A Rabbit polyclonal antibody (STJ26241) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-UBE4A antibody (797-1066) (STJ26241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human USP14 protein (Recombinant) (N-His) (STJP014536)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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Cullin 4A antibody western blot HL-60 U2OS STJA0035171

Anti-Cullin 4A antibody [R06-0C5] (STJA0035171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of 293T, using MARCH9 antibody (STJ112609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MARCH9 antibody (277-346) (STJ112609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TRIM2 antibody (162-212 aa) (STJ193894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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SDS-PAGE gel showing recombinant human UBTD2 protein stained with Coomassie blue

Human UBTD2 protein (Recombinant) (His-Tag) (STJP017806)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Human UBE2S protein (Recombinant) (N-GST) (STJP014650)

Host :

E.coli

Applications :

ELISA/WB

£319.50

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Anti-UBE2L6 antibody [SRM14696] (STJ11109426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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