| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-UBE3A (300-590) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBE3A |
| Gene ID: |
7337 |
| Uniprot ID: |
UBE3A_HUMAN |
| Immunogen Region: |
300-590 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human UBE3A (NP_570853.1). |
| Immunogen Sequence: |
KLIRLWSKYNADQIRRMMET FQQLITYKVISNEFNSRNLV NDDDAIVAASKCLKMVYYAN VVGGEVDTNHNEEDDEEPIP ESSELTLQELLGEERRNKKG PRVDPLETELGVKTLDCRKP LIPFEEFINEPLNEVLEMDK DYTFFKVETENKFSFMTCPF ILNAVTKNLGLYYDNRIRMY SERRITVLYSLVQGQQLNPY LRLKVRRDHIIDDALVRLEM IAMENPADLKKQLYVEFEG |
| Post Translational Modifications | Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation. Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation. Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC. Required for synaptic remodeling in neurons by mediating ubiquitination and degradation of LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling. Synergizes with WBP2 in enhancing PGR activity. (Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. |
| Protein Name | Ubiquitin-Protein Ligase E3aE6ap Ubiquitin-Protein LigaseHect-Type Ubiquitin Transferase E3aHuman Papillomavirus E6-Associated ProteinOncogenic Protein-Associated Protein E6-ApRenal Carcinoma Antigen Ny-Ren-54 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Ubiquitin-Protein Ligase E3a antibodyAnti-E6ap Ubiquitin-Protein Ligase antibodyAnti-Hect-Type Ubiquitin Transferase E3a antibodyAnti-Human Papillomavirus E6-Associated Protein antibodyAnti-Oncogenic Protein-Associated Protein E6-Ap antibodyAnti-Renal Carcinoma Antigen Ny-Ren-54 antibodyAnti-UBE3A antibodyAnti-E6AP antibodyAnti-EPVE6AP antibodyAnti-HPVE6A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
