• Western blot analysis of extracts of various cell lines, using HERC3 antibody (STJ111302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using HERC3 antibody (STJ111302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HERC3 antibody (1-200) (STJ111302)

SKU:
STJ111302

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HERC3 (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HERC3
Gene ID: 8916
Uniprot ID: HERC3_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HERC3 (NP_055421.1).
Immunogen Sequence: MLCWGYWSLGQPGISTNLQG IVAEPQVCGFISDRSVKEVA CGGNHSVFLLEDGEVYTCGL NTKGQLGHEREGNKPEQIGA LADQHIIHVACGESHSLALS DRGQLFSWGAGSDGQLGLMT TEDSVAVPRLIQKLNQQTIL QVSCGNWHCLALAADGQFFT WGKNSHGQLGLGKEFPSQAS PQRVRSLEGIPLAQVAAGGA
Post Translational Modifications Ubiquitinated.which promotes degradation by the proteasome.
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Protein Name Probable E3 Ubiquitin-Protein Ligase Herc3
Hect Domain And Rcc1-Like Domain-Containing Protein 3
Hect-Type E3 Ubiquitin Transferase Herc3
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cytoplasmic Vesicle
Also Found In Vesicular-Like Structures
Alternative Antibody Names Anti-Probable E3 Ubiquitin-Protein Ligase Herc3 antibody
Anti-Hect Domain And Rcc1-Like Domain-Containing Protein 3 antibody
Anti-Hect-Type E3 Ubiquitin Transferase Herc3 antibody
Anti-HERC3 antibody
Anti-KIAA0032 antibody

Information sourced from Uniprot.org

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