• Immunohistochemistry analysis of paraffin-embedded rat brain using UBE4A Rabbit polyclonal antibody (STJ26241) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using UBE4A antibody (STJ26241) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-UBE4A antibody (797-1066) (STJ26241)

SKU:
STJ26241

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBE4A (797-1066) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE4A
Gene ID: 9354
Uniprot ID: UBE4A_HUMAN
Immunogen Region: 797-1066
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 797-1066 of human UBE4A (NP_001191006.1).
Immunogen Sequence: FLLDEAIQYLSKIKIQQIEK DRGEWDSLTPEARREKEAGL QMFGQLARFHNIMSNETIGT LAFLTSEIKSLFVHPFLAER IISMLNYFLQHLVGPKMGAL KVKDFSEFDFKPQQLVSDIC TIYLNLGDEENFCATVPKDG RSYSPTLFAQTVRVLKKINK PGNMIMAFSNLAERIKSLAD LQQQEEETYADACDEFLDPI MSTLMCDPVVLPSSRVTVDR STIARHLLSDQTDPFNRSP
Function Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.
Protein Name Ubiquitin Conjugation Factor E4 A
Ring-Type E3 Ubiquitin Transferase E4 A
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ubiquitin Conjugation Factor E4 A antibody
Anti-Ring-Type E3 Ubiquitin Transferase E4 A antibody
Anti-UBE4A antibody
Anti-KIAA0126 antibody

Information sourced from Uniprot.org

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