Transport Of Small Molecules | Research Area | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded human heart, using Dematin (Phospho-Ser403) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-DMTN-Ser403 antibody (356-405 aa) (STJ90897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-Phospho-eIF2A-Ser51 antibody [R07-5H9] (STJA0033925)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of lysates from HeLa cells, using Phospho-eIF2 Alpha-S49 Rabbit pAb (STJ22112). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-EIF2S1-S49 antibody (STJ22112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells, using Phospho-eIF2 Alpha-S51 Rabbit pAb (STJ23509). HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-EIF2S1-S51 antibody (STJ116376)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various lysates using Phospho-eIF2 Alpha-S51 Rabbit mAb (STJ11102562) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-EIF2S1-S51 antibody [S2MR] (STJ11102562)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-eIF2α (Phospho-Ser51) (PTR1316) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 40kDaObserved band size: 40, 90kDa

Anti-Phospho-EIF2S1-Ser51 antibody (1-100) [PTR1316] (STJA0006271)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha (phospho Ser51) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-EIF2S1-Ser51 antibody (21-70 aa) (STJ90253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Dog/Pig/Fish

$67.62 - $360.18

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Anti-Phospho-EIF2S2-Ser67 antibody (STJA0009866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-KCNJ11-Thr224 antibody (190-239 aa) (STJ90722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-MLKL antibody (350-400) (STJ506097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-Phospho-MLKL antibody (350-400) {Biotin} (STJ506098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Anti-Phospho-MLKL antibody (350-400) {FITC} (STJ506099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$761.07

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Anti-Phospho-MLKL-S358 antibody (STJ11108414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MLKL-S358 antibody (STJ193224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, phospho-MLKL (S358) Mouse monoclonal antibody (6F8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-MLKL-S358 antibody [6F8] (STJ97776)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human

$67.62 - $360.18

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Anti-Phospho-MLKL-Ser345 antibody [PT0987R] (STJA0039926)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-Phospho-MLKL-Ser358 antibody [R02-2I5] (STJA0036596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

$184.23 - $388.47

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Western blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (STJ11100984) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-NEDD4L-S448 antibody (STJ11100984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western Blot analysis of 1 Hela treated with LPS, 2 Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NEDD4L-Ser342 antibody (STJ196363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-NEDD4L-Ser448 antibody (STJ196364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

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Anti-Phospho-Phospholamban-Ser16 antibody [R03-1I4] (STJA0034720)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$184.23 - $388.47

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Anti-Phospho-PKA RII alpha-Ser99 antibody [R05-7G2] (STJA0034728)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of various lysates using Phospho-PLN-S16 Rabbit pAb (STJ11101042) at 1:1000 dilution. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PLN-S16 antibody (STJ11101042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Phospho-PLN-S16/T17 Rabbit pAb (STJ11103720) at 1:1000 dilution. Hela cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PLN-S16/T17 antibody (STJ11103720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of HUVEC cells, using PLB (Phospho-Ser16+Thr17) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PLN-Ser16/T17 antibody (1-50 aa) (STJ91324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from HeLa cells, using Phospho-PLN-T17 Rabbit pAb (STJ11101045) at 1:400 dilution. HeLa cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-Phospho-PLN-T17 antibody (STJ11101045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from HeLa and 293 cells, using Phospho-PKA C-alpha (PRKACA) -S339 Rabbit pAb (STJ113496) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKACA-S339 antibody (STJ113496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-PRKACA-Ser338 antibody (STJ91123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from 293T cells, using Phospho-PKA C-alpha (PRKACA) -T197 Rabbit pAb (STJ113495) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKACA-T197 antibody (STJ113495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from 293T cells, using Phospho-PKA RII Alpha (PRKAR2A) -S99 Rabbit mAb (STJ11102613) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PRKAR2A-S99 antibody [S3MR] (STJ11102613)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKAR2B-Ser113 antibody (79-128 aa) (STJ90520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

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Western blot analysis of 3T3 cells using Phospho-20S Proteasome Alpha 3 (S250) Polyclonal Antibody

Anti-Phospho-PSMA3-Ser250 antibody (206-255 aa) (STJ90556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (STJ113482) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RAF1-S259 antibody (STJ113482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb (STJ11102598) at 1:1000 dilution. Both 293T cells and C6 cells were treated by Forskolin (10 uM) at 37 °C for 1 hour after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RAF1-S259 antibody [S8MR] (STJ11102598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Raf1-S338 pAb (STJ117871) at 1:1000 dilution or Phospho-Raf1-S338 antibody (STJ25284). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-RAF1-S338 antibody (STJ117871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa and 293T cells, using Phospho-Raf1-S621 Rabbit pAb (STJ22366) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA (200nM) for 30 minutes or treated by EGF (25 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RAF1-S621 antibody (STJ22366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of lysates from C6 cells, using Phospho-Raf1-S621 Rabbit mAb (STJ11102597) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-RAF1-S621 antibody [S7MR] (STJ11102597)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Anti-Phospho-RAF1-Ser-471 [S578/S433] antibody (STJA0005562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$507.15

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Western blot analysis of various cells using Phospho-Raf-1 (S259) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-RAF1-Ser259 antibody (225-274 aa) (STJ90399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Anti-Phospho-Raf1-Ser259 antibody [R07-4J4] (STJA0034791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunohistochemistry analysis of paraffin-embedded human brain, using C-RAF (Phospho-Ser289) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Ser289 antibody (251-300 aa) (STJ91218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of 293 cells using Phospho-Raf-1 (S296) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-RAF1-Ser296 antibody (271-320 aa) (STJ90850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of A549 cells using Phospho-Raf-1 (S301) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-RAF1-Ser301 antibody (STJ90595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various cells using Phospho-Raf-1 (S338) Polyclonal Antibody

Anti-Phospho-RAF1-Ser338 antibody (305-354 aa) (STJ90676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from NIH/3T3 cells, using C-RAF (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Ser43 antibody (11-60 aa) (STJ90513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Raf1-Ser43 antibody [R09-2I1] (STJA0036210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RAF1-Ser621 antibody (591-640 aa) (STJ91052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Anti-Phospho-Raf1-Ser621 antibody [R09-8G1] (STJA0036194)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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