• Western blot analysis of extracts of various cell lines, using Phospho-PLN-S16/T17 antibody (STJ11103720) at 1:1000 dilution. Hela cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using Phospho-PLN-S16/T17 Rabbit polyclonal antibody (STJ11103720) at 1:1000 dilution. Hela cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-PLN-S16/T17 antibody (STJ11103720)

SKU:
STJ11103720

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PLN-S16/T17 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLN
Gene ID: 5350
Uniprot ID: PPLA_HUMAN
Immunogen: A synthetic phosphorylated peptide around S16 & T17 of human PLN (NP_002658.1).
Immunogen Sequence: ASTIE
Tissue Specificity Heart muscle (at protein level).
Post Translational Modifications Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. Palmitoylated by ZDHHC16, promoting formation of the homopentamer. In elongated spermatids, proteolytically cleaved by SPPL2C which modulates intracellular Ca(2+) homeostasis.
Function Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. Controls intracellular Ca(2+) levels in elongated spermatids. May play a role in germ cell differentiation.
Protein Name Cardiac Phospholamban
Plb
Database Links Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Sarcoplasmic Reticulum Membrane
Mitochondrion Membrane
Membrane
Colocalizes With Hax1 At The Endoplasmic Reticulum
Colocalizes With Dmpk A The Sarcoplasmic Reticulum
Alternative Antibody Names Anti-Cardiac Phospholamban antibody
Anti-Plb antibody
Anti-PLN antibody
Anti-PLB antibody

Information sourced from Uniprot.org

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