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Anti-Phospho-PSMA3-Ser250 antibody (206-255 aa) (STJ90556)

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using Proteasome alpha3 (Phospho-Ser250) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of 3T3 cells using Phospho-20S Proteasome Alpha 3 (S250) Polyclonal Antibody

Anti-Phospho-PSMA3-Ser250 antibody (206-255 aa) (STJ90556)

SKU:

STJ90556

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Proteasome subunit alpha type-3-Ser250 (206-255 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PSMA3
Gene ID:	5684
Uniprot ID:	PSA3_HUMAN
Immunogen Region:	206-255 aa
Specificity:	Phospho-20S Proteasome Alpha 3 (S250) Polyclonal Antibody detects endogenous levels of 20S Proteasome Alpha 3 protein only when phosphorylated at S250.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Proteasome alpha3 around the phosphorylation site of Ser250 at the amino acid range 206-255

Protein Name	Proteasome Subunit Alpha Type-3 Macropain Subunit C8 Multicatalytic Endopeptidase Complex Subunit C8 Proteasome Component C8
Database Links	Reactome: R-HSA-1169091 Reactome: R-HSA-1234176 Reactome: R-HSA-1236974 Reactome: R-HSA-1236978 Reactome: R-HSA-174084 Reactome: R-HSA-174113 Reactome: R-HSA-174154 Reactome: R-HSA-174178 Reactome: R-HSA-174184 Reactome: R-HSA-180534 Reactome: R-HSA-180585 Reactome: R-HSA-187577 Reactome: R-HSA-195253 Reactome: R-HSA-202424 Reactome: R-HSA-211733 Reactome: R-HSA-2467813 Reactome: R-HSA-2871837 Reactome: R-HSA-349425 Reactome: R-HSA-350562 Reactome: R-HSA-382556 Reactome: R-HSA-450408 Reactome: R-HSA-4608870 Reactome: R-HSA-4641257 Reactome: R-HSA-4641258 Reactome: R-HSA-5358346 Reactome: R-HSA-5362768 Reactome: R-HSA-5607761 Reactome: R-HSA-5607764 Reactome: R-HSA-5610780 Reactome: R-HSA-5610783 Reactome: R-HSA-5610785 Reactome: R-HSA-5632684 Reactome: R-HSA-5658442 Reactome: R-HSA-5668541 Reactome: R-HSA-5676590 Reactome: R-HSA-5678895 Reactome: R-HSA-5687128 Reactome: R-HSA-5689603 Reactome: R-HSA-5689880 Reactome: R-HSA-68867 Reactome: R-HSA-68949 Reactome: R-HSA-69017 Reactome: R-HSA-69481 Reactome: R-HSA-69601 Reactome: R-HSA-75815 Reactome: R-HSA-8852276 Reactome: R-HSA-8854050 Reactome: R-HSA-8939236 Reactome: R-HSA-8939902 Reactome: R-HSA-8941858 Reactome: R-HSA-8948751 Reactome: R-HSA-8951664 Reactome: R-HSA-9010553 Reactome: R-HSA-9020702 Reactome: R-HSA-9604323 Reactome: R-HSA-9755511 Reactome: R-HSA-9762114 Reactome: R-HSA-9824272 Reactome: R-HSA-983168
Cellular Localisation	Cytoplasm Nucleus Translocated From The Cytoplasm Into The Nucleus Following Interaction With Akirin2 Which Bridges The Proteasome With The Nuclear Import Receptor Ipo9
Alternative Antibody Names	Anti-Proteasome Subunit Alpha Type-3 antibody Anti-Macropain Subunit C8 antibody Anti-Multicatalytic Endopeptidase Complex Subunit C8 antibody Anti-Proteasome Component C8 antibody Anti-PSMA3 antibody Anti-HC8 antibody Anti-PSC8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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