Signal Transduction | Research Area | St John's Laboratory

Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2 (ABT077) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SK-BR-3Predicted band size: 137kDaObserved band size: 200kDa

Anti-ERBB2 antibody (300-400 aa) [ABT077] (STJ197163)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2 (ABT338) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 137kDaObserved band size: 200kDa

Anti-ERBB2 antibody (300-400 aa) [ABT338] (STJ197087)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various cells using Neu Polyclonal Antibody diluted at 1：2000

Anti-ERBB2 antibody (641-690) (STJ94412)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Neu monoclonal antibody against SKBR3 (1) and MCF-7 (2) cell lysate.

Anti-ERBB2 antibody (750-987 aa) [6C2] (STJ98272)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using Neu monoclonal antibody against truncated Neu recombinant protein.

Anti-ERBB2 antibody (750-987 aa) [6C2B12/9B9D8] (STJ98271)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Human breast infiltrating ductal carcinoma stained with anti-Her-2/neu antibody using peroxidase-conjugate and DAB chromogen. Note the strong membrane staining (3+) of carcinoma cells.

Anti-ERBB2 antibody (ECD) [ZR5] (STJ180123)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£200.50 - £842.50

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Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ERBB2 antibody [11H9] (STJ96947)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 293, 3) Jurkat, diluted at 1:2000.

Anti-ERBB2 antibody [3C9] (STJ96981)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot detection of HER2/ErbB2 fragment in CHO-K1 cell lysate (B) and CHO-K1 transfected by pEGFP-C1-HER2/ErbB2 (A) cell lysate using HER2/ErbB2 mouse mAb (1:1000 diluted).Predicted band size: 55kDa.Observed band size:55kDa.

Anti-ERBB2 antibody [5B6-A9-C8] (STJ99273)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Transfected

£144.00 - £348.00

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Anti-ERBB2 antibody [9B9D8] (STJ98270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of ERBB2 at dilution of 1:1000

Anti-ErbB2 antibody [SM0478] (STJA0010478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of various lysates using ERBB2IP Rabbit pAb (STJ111318) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ERBB2IP antibody (1152-1371) (STJ111318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ERBIN antibody (STJA0010177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-ERCC6L antibody (STJA0007388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver, antibody was diluted at 1:100

Anti-EREG antibody (71-120 aa) (STJ99008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates, using EREG rabbit polyclonal antibody (STJ118800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EREG antibody (C-Term) (STJ118800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-EREG/Epiregulin antibody [SAA1501] (STJA0019929)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Anti-ERK5 antibody [4D6] (STJ196684)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-ERK5 antibody [4G5] (STJ196678)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human KidneyTissue using ERK5 mouse Monoclonal antibody diluted at 1:200.

Anti-ERK5 antibody [6D10] (STJ196698)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-Erythroid Alpha-Spectrin antibody [AF10] (STJ16100820)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB/ICC

Reactivity :

Human

£606.50

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Human kidney stained with anti-Erythropoietin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tubular cells.

Anti-Erythropoietin antibody (28-162aa) [ZM135] (STJ180351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£176.50 - £624.50

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Human colon adenocarcinoma stained with MOC-31 antibody using peroxidase-conjugate and DAB chromogen. Note the membranous staining of tumor cells.

Anti-ESA antibody [MOC‐31] (STJ180098)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £525.50

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Anti-ESR Alpha antibody [SRM14746] (STJ11109476)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP/FC

Reactivity :

Human

£0.00

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Anti-ESR1 antibody (1-190) (STJ119086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 using ESR Alpha Rabbit monoclonal antibody (STJ11104746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ESR1 antibody (100-200) [S4746RM] (STJ11104746)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using ER Alpha Polyclonal Antibody diluted at 1：500

Anti-ESR1 antibody (136-185 aa) (STJ92997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot with Anti-ESR1 Antibody (STJ500969) and peptide at 1:500 dilution in buffer.

Anti-ESR1 antibody (30-80) (STJ500969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£427.50

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Anti-ESR1 antibody (30-80) {Biotin} (STJ500972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-ESR1 antibody (30-80) {FITC} (STJ500973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£528.50

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Human breast carcinoma tissue was stained with Anti-Estrogen Receptor α (PTR2124) Antibody

Anti-ESR1 antibody (450-595) [PTR2124] (STJA0006244)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of lysates from MCF7 cells, using ESR Alpha Rabbit monoclonal antibody (STJ114945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-ESR1 antibody (50-150) [S5MR] (STJ114945)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ESR1 antibody (501-550 aa) (STJ92999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using ESR Alpha antibody (STJ23572) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ESR1 antibody (516-595) (STJ23572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-ESR1 antibody (520-570) (STJ500970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£427.50

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Anti-ESR1 antibody (520-570) {Biotin} (STJ500974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£528.50

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Anti-ESR1 antibody (520-570) {FITC} (STJ500975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£528.50

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Immunohistochemistry of mouse liver with Anti-ESR1 primary antibody (STJ500971) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ESR1 antibody (545-595) (STJ500971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-ESR1 antibody (545-595) {Biotin} (STJ500976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-ESR1 antibody (545-595) {FITC} (STJ500977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-ESR1 antibody (C-Term) (STJA0005414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of 1) Hela, 2) MCF7, 3) Mouse Liver Tissue, 4) Rat Liver Tissue with Estrogen Receptor a Rabbit pAb diluted at 1:2,000.

Anti-ESR1 antibody (STJ197393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using ER monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ER cDNA (2).

Anti-ESR1 antibody [5D4B1/5D4E3] (STJ98040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using ER Alpha monoclonal antibody against MCF-7 (1) , T47D (2) and SKBR3 (3) cell lysate.

Anti-ESR1 antibody [6B6] (STJ98054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-ESR1/ER-alpha antibody [RB125] (STJA0022317)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF

Reactivity :

Human

£378.50 - £1,632.50

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Anti-ESR1/ER-alpha antibody [RB133] (STJA0022318)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of lysates from Daudi cells using Estrogen Receptor beta Rabbit polyclonal antibody (STJ23576) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ESR2 antibody (1-153) (STJ23576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-ER-β (PT0318) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HEK293 Lane 3: MCF7Predicted band size: 59kDaObserved band size: 59kDa

Anti-ESR2 antibody (400-530 aa) (STJ196169)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£48.00 - £252.00

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