Anti-DGKE antibody (161-210 aa) (STJ92699)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human DGKE at the amino acid range 161-210
General Information
| Short Description | Rabbit polyclonal anti-Diacylglycerol kinase epsilon (161-210 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DGKE |
| Gene ID | 8526 |
| Uniprot ID | DGKE_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human DGKE at the amino acid range 161-210 |
| Immunogen Region | 161-210 aa |
| Specificity | DGK-Epsilon Polyclonal Antibody detects endogenous levels of DGK-Epsilon protein. |
Additional Info
| Function | Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently. |
| Protein Name | Diacylglycerol Kinase EpsilonDag Kinase EpsilonDiglyceride Kinase EpsilonDgk-Epsilon |
| Database Links | Reactome: R-HSA-114508 |
| Cellular Localisation | MembraneSingle-Pass Membrane ProteinCytoplasm |
| Alternative Antibody Names | Anti-Diacylglycerol Kinase Epsilon antibodyAnti-Dag Kinase Epsilon antibodyAnti-Diglyceride Kinase Epsilon antibodyAnti-Dgk-Epsilon antibodyAnti-DGKE antibodyAnti-DAGK5 antibody |
Information sourced from Uniprot.org