• Immunofluorescence analysis of HeLa cells, using DGKI Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from 293 cells, using DGKI Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using DGK-Iota Polyclonal Antibody diluted at 1：1000

Anti-DGKI antibody (991-1040 aa) (STJ92703)

SKU:
STJ92703

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Diacylglycerol kinase iota (991-1040 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DGKI
Gene ID: 9162
Uniprot ID: DGKI_HUMAN
Immunogen Region: 991-1040 aa
Specificity: DGK-Iota Polyclonal Antibody detects endogenous levels of DGK-Iota protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DGKI at the amino acid range 991-1040
Function Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position. By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway. May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression.
Protein Name Diacylglycerol Kinase Iota
Dag Kinase Iota
Dgk-Iota
Database Links Reactome: R-HSA-114508
Cellular Localisation Cell Projection
Axon
Dendrite
Presynapse
Postsynapse
Postsynaptic Density
Synaptic Cell Membrane
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Cytoplasm
Cytosol
Nucleus
Excluded From Inhibitory Synapses
Localization Between Cytoplasm And Nucleus Is Regulated By Protein Kinase C
Both In The Detergent Soluble And Particulate Fractions
Alternative Antibody Names Anti-Diacylglycerol Kinase Iota antibody
Anti-Dag Kinase Iota antibody
Anti-Dgk-Iota antibody
Anti-DGKI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance