Anti-DDX4 antibody [SRM14972] (STJ11109702)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal DDX4 antibody for use in WB, IP, IF-P, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/IF-P/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14972 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:5000-1:30000IP, 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsIF-P, 1:200-1:800IHC-P, 1:700-1:7000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on you |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DDX4 |
| Gene ID | 54514 |
| Uniprot ID | DDX4_HUMAN |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. |
| Function | ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs. |
| Protein Name | Probable Atp-Dependent Rna Helicase Ddx4Dead Box Protein 4Vasa Homolog |
| Database Links | Reactome: R-HSA-5601884Reactome: R-HSA-9696264 |
| Cellular Localisation | CytoplasmPerinuclear RegionComponent Of The Meiotic NuageAlso Named P GranuleA Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis |
| Alternative Antibody Names | Anti-Probable Atp-Dependent Rna Helicase Ddx4 antibodyAnti-Dead Box Protein 4 antibodyAnti-Vasa Homolog antibodyAnti-DDX4 antibodyAnti-VASA antibody |
Information sourced from Uniprot.org