| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DDX5 (1-614) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DDX5 |
| Gene ID: |
1655 |
| Uniprot ID: |
DDX5_HUMAN |
| Immunogen Region: |
1-614 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human DDX5 (NP_004387.1). |
| Immunogen Sequence: |
MSGYSSDRDRGRDRGFGAPR FGGSRAGPLSGKKFGNPGEK LVKKKWNLDELPKFEKNFYQ EHPDLARRTAQEVETYRRSK EITVRGHNCPKPVLNFYEAN FPANVMDVIARQNFTEPTAI QAQGWPVALSGLDMVGVAQT GSGKTLSYLLPAIVHINHQP FLERGDGPICLVLAPTRELA QQVQQVAAEYCRACRLKSTC IYGGAPKGPQIRDLERGVEI CIATPGRLIDFLECGKTNL |
| Post Translational Modifications | Arg-502 is dimethylated, probably to asymmetric dimethylarginine. Sumoylated.sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination. Polyubiquitinated, leading to proteasomal degradation. Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues. |
| Function | Involved in the alternative regulation of pre-mRNA splicing.its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation.the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner.the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. |
| Protein Name | Probable Atp-Dependent Rna Helicase Ddx5Dead Box Protein 5Rna Helicase P68 |
| Database Links | Reactome: R-HSA-3899300Reactome: R-HSA-72163Reactome: R-HSA-9018519Reactome: R-HSA-9682706Reactome: R-HSA-9694686 |
| Cellular Localisation | NucleusNucleolusNucleus SpeckleCytoplasmDuring The G0 PhasePredominantly Located In The NucleusCytoplasmic Levels Increase During The G1/S PhaseDuring The M PhaseLocated At The Vicinity Of The Condensed ChromosomesAt G1Localizes In The Cytoplasm |
| Alternative Antibody Names | Anti-Probable Atp-Dependent Rna Helicase Ddx5 antibodyAnti-Dead Box Protein 5 antibodyAnti-Rna Helicase P68 antibodyAnti-DDX5 antibodyAnti-G17P1 antibodyAnti-HELR antibodyAnti-HLR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
