Programmed Cell Death | Research Area | St John's Laboratory

Immunofluorescence analysis of un-treated (left) A549 and UV treated (right) A549 cell. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-GSDMD antibody (1-100) (STJA0010253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit monoclonal antibody (STJ11105210) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-GSDMD antibody (276-484) [S5210RM] (STJ11105210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €718.13

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GSDMD antibody (STJ11100237). Western blot was performed from the immunoprecipitate using GSDMD antibody (STJ11100237) at a dilution of 1:500.

Anti-GSDMD antibody (346-484) (STJ11100237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of U2OS cells using GSDMD Rabbit polyclonal antibody (STJ119438) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSDMD antibody (346-484) (STJ119438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit monoclonal antibody (STJ11103428) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-GSDMD antibody (346-484) [S8MR] (STJ11103428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Anti-GSDMD antibody (AA range: 1-100) [PTR1398] (STJA0038893)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of lysates from THP-1 cells, using GSDMD (Full Length+N terminal) Rabbit pAb (STJ11105919) at 1:400 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GSDMD antibody (Full Length + N-Term) (STJ11105919)

Clonality :

Polyclonal

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates, using GSDMD (Full Length+N terminal) Rabbit pAb (STJ11106595) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-GSDMD antibody (Full Length + N-Term) (STJ11106595)

Clonality :

Polyclonal

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of GSDMD in Jurkat lysates using GSDMD antibody.

Anti-GSDMD antibody (Full-length) [R06-5C5] (STJA0013051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€330.63

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Anti-GSDMD antibody [SAA1652] (STJA0020080)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€426.88 - €1,809.38

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Anti-GSDME antibody (1-100) (STJA0010252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of DFNA5/GSDME in U251MG, U2OS, HepG2, Hela lysates using GSDME antibody.

Anti-GSDME antibody (1-270 aa) [R07-5I1] (STJA0011231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

€308.13

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Western blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GSDME antibody (200-280 aa) (STJ96411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using GSDME Rabbit pAb (STJ29568) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GSDME antibody (271-496) (STJ29568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from 1) Mouse-brain, 2) Hela cells treated by UV 15min, 3) HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody HRP goat anti rabbit (STJ99666) was diluted at 1:10000

Anti-GSDME antibody (400-496) (STJA0010251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of Gelsolin in paraffin-embedded human tonsil using Gelsolin Rabbit polyclonal antibody (STJ11104659) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSN antibody (28-782) (STJ11104659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunofluorescence analysis of U-2 OS cells using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSN antibody (282-438) (STJ11101188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of paraffin-embedded mouse brain using Gelsolin Rabbit monoclonal antibody (STJ11101830) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSN antibody (400-500) [S0MR] (STJ11101830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€161.88 - €718.13

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Western Blot analysis of varius cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-GSN antibody (STJ192165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-GSN nanobody [SAA0980] (STJN000110)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Anti-GSN nanobody [SAA1010] (STJN000111)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Anti-GSN nanobody [SAA1011] (STJN000112)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Anti-GSN nanobody [SAA1179] (STJN000109)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF

Reactivity :

Human

€473.13 - €2,040.63

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Immunohistochemistry of rat spleen with Anti-GZMB primary antibody (STJ501289) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GZMB antibody (1-50) (STJ501289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

€534.38

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Anti-GZMB antibody (1-50) {Biotin} (STJ501291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Rat

€660.63

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Anti-GZMB antibody (1-50) {FITC} (STJ501292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Rat

€660.63

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Anti-GZMB antibody (100-200 aa) [PT0564] (STJ196705)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

€217.50 - €315.00

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Immunofluorescence analysis of mouse spleen cells using Granzyme B Rabbit polyclonal antibody (STJ26211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GZMB antibody (100-200) (STJ26211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry of baboon spleen with Anti-GZMB primary antibody (STJ501290) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-GZMB antibody (50-100) (STJ501290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€534.38

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Anti-GZMB antibody (50-100) {Biotin} (STJ501293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€660.63

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Anti-GZMB antibody (50-100) {FITC} (STJ501294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€660.63

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Western blot analysis of mouse-lung lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-GZMB antibody (81-130 aa) (STJ99635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-GZMB antibody [Fv02] (STJA0027022)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€318.13 - €473.13

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Anti-GZMB antibody [Fv02] {APC} (STJA0029113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€388.13 - €590.63

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Anti-GZMB antibody [Fv02] {FITC} (STJA0028147)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€318.13 - €473.13

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Anti-GZMB antibody [Fv02] {PerCP} (STJA0030079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€365.63 - €550.63

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Anti-GZMB antibody [Fv02] {PE} (STJA0031045)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€388.13 - €590.63

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Anti-GZMB antibody [SAA0515] (STJA0018519)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

€473.13 - €2,040.63

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Anti-GZMB antibody [SAA0516] (STJA0019362)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/IHC/WB

Reactivity :

Human

€473.13 - €2,040.63

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Anti-GZMB antibody [scFv17] (STJA0027021)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€318.13 - €473.13

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Anti-GZMB antibody [scFv17] {APC} (STJA0029114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€388.13 - €590.63

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Anti-GZMB antibody [scFv17] {FITC} (STJA0028148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€318.13 - €473.13

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Anti-GZMB antibody [scFv17] {PerCP} (STJA0030080)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€365.63 - €550.63

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Anti-GZMB antibody [scFv17] {PE} (STJA0031046)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

€388.13 - €590.63

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Western blot analysis of various cells using Histone 1.0 Polyclonal Antibody diluted at 1：2000

Anti-H1-0 antibody (71-120 aa) (STJ93509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H1-1 antibody (49-99 aa) (STJ195717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of U2OS cells using HIST1H1C Rabbit polyclonal antibody (STJ11105680) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-H1-2 antibody (100-200) (STJ11105680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-HIST1H1D antibody (STJA0004374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

€175.63 - €519.38

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