• Immunohistochemistry analysis of Smac/Diablo in paraffin-embedded mouse testis using Smac/Diablo Rabbit polyclonal antibody (STJ23380) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Smac/Diablo in paraffin-embedded rat testis using Smac/Diablo Rabbit polyclonal antibody (STJ23380) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Smac/Diablo Rabbit polyclonal antibody (STJ23380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-DIABLO antibody (56-239) (STJ23380)

SKU:
STJ23380

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DIABLO (56-239) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DIABLO
Gene ID: 56616
Uniprot ID: DBLOH_HUMAN
Immunogen Region: 56-239
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 56-239 of human Smac/Diablo (NP_063940.1).
Immunogen Sequence: AVPIAQKSEPHSLSSEALMR RAVSLVTDSTSTFLSQTTYA LIEAITEYTKAVYTLTSLYR QYTSLLGKMNSEEEDEVWQV IIGARAEMTSKHQEYLKLET TWMTAVGLSEMAAEAAYQTG ADQASITARNHIQLVKLQVE EVHQLSRKAETKLAEAQIEE LRQKTQEEGEERAESEQEAY LRED
Tissue Specificity Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.
Post Translational Modifications Ubiquitinated by BIRC7/livin. The precursor form is proteolytically cleaved by mitochondrial processing peptidase MPP to remove the transit peptide and produce an intermediate form. This is then processed by PARL to produce the mature cleaved form which is released from mitochondria into the cytosol in apoptotic cells.
Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3: Attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1: Defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Protein Name Diablo Iap-Binding Mitochondrial Protein
Diablo Homolog - Mitochondrial
Direct Iap-Binding Protein With Low Pi
Second Mitochondria-Derived Activator Of Caspase
Smac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form
Database Links Reactome: R-HSA-111457
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-111469
Reactome: R-HSA-9627069
Cellular Localisation Mitochondrion
Cytoplasm
Cytosol
Released Into The Cytosol In A Parl-Dependent Manner When Cells Undergo Apoptosis
Alternative Antibody Names Anti-Diablo Iap-Binding Mitochondrial Protein antibody
Anti-Diablo Homolog - Mitochondrial antibody
Anti-Direct Iap-Binding Protein With Low Pi antibody
Anti-Second Mitochondria-Derived Activator Of Caspase antibody
Anti-Smac Cleaved Into - Diablo Iap-Binding Mitochondrial Protein - Cleaved Form antibody
Anti-DIABLO antibody
Anti-SMAC antibody

Information sourced from Uniprot.org

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