Anti-Desmoglein 3 antibody (STJA0037776)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human Desmoglein 3
General Information
| Short Description | Rabbit monoclonal anti-Desmoglein 3 for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | IHC-P-1:100-1:200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DSG3 |
| Gene ID | 1830 |
| Uniprot ID | DSG3_HUMAN |
| Immunogen | Recombinant protein of human Desmoglein 3 |
Additional Info
| Tissue Specificity | Expressed throughout the basal and spinous layer of the epidermis with weak expression in the granular layer (at protein level). Expressed in skin and mucosa (at protein level). Expressed in the basal layer of the outer root sheath of the telogen hair club, specifically at the cell membrane between the apex of the cells and the surrounding hair club (at protein level). Expression is less abundant between the lateral margins of the outer root sheath basal cells (at protein level). Also expressed in the tongue, tonsil and esophagus. |
| Function | A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle. Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells. May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3. Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG. May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation. Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1. The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity. Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing. |
| Protein Name | Desmoglein-3130 Kda Pemphigus Vulgaris AntigenPvaCadherin Family Member 6 |
| Database Links | Reactome: R-HSA-351906Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionDesmosomeCytoplasmTight Junction |
| Alternative Antibody Names | Anti-Desmoglein-3 antibodyAnti-130 Kda Pemphigus Vulgaris Antigen antibodyAnti-Pva antibodyAnti-Cadherin Family Member 6 antibodyAnti-DSG3 antibodyAnti-CDHF6 antibody |
Information sourced from Uniprot.org