Anti-DAPK2 antibody [R09-8F2] (STJA0034232)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human DAPK2
General Information
| Short Description | Rabbit monoclonal anti-DAPK2 for use in WB and IHC-P in Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R09-8F2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DAPK2 |
| Gene ID | 23604 |
| Uniprot ID | DAPK2_HUMAN |
| Immunogen | A synthetic peptide of human DAPK2 |
Additional Info
| Tissue Specificity | Expressed in neutrophils and eosinophils. Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. |
| Post Translational Modifications | Autophosphorylation at Ser-318 inhibits its catalytic activity. Dephosphorylated at Ser-318 in response to activated Fas and TNF-alpha receptors. |
| Function | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Regulates granulocytic motility by controlling cell spreading and polarization. Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death. |
| Protein Name | Death-Associated Protein Kinase 2Dap Kinase 2Dap-Kinase-Related Protein 1Drp-1 |
| Database Links | Reactome: R-HSA-418889 |
| Cellular Localisation | CytoplasmCytoplasmic VesicleAutophagosome Lumen |
| Alternative Antibody Names | Anti-Death-Associated Protein Kinase 2 antibodyAnti-Dap Kinase 2 antibodyAnti-Dap-Kinase-Related Protein 1 antibodyAnti-Drp-1 antibodyAnti-DAPK2 antibody |
Information sourced from Uniprot.org