• Western blot analysis of extracts of various cell lines, using DFFB antibody (STJ112149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DFFB antibody (109-338) (STJ112149)

SKU:
STJ112149

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DFFB (109-338) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DFFB
Gene ID: 1677
Uniprot ID: DFFB_HUMAN
Immunogen Region: 109-338
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1).
Immunogen Sequence: DEQAPQRQRLLADLLHNVSQ NIAAETRAEDPPWFEGLESR FQSKSGYLRYSCESRIRSYL REVSSYPSTVGAEAQEEFLR VLGSMCQRLRSMQYNGSYFD RGAKGGSRLCTPEGWFSCQG PFDMDSCLSRHSINPYSNRE SRILFSTWNLDHIIEKKRTI IPTLVEAIKEQDGREVDWEY FYGLLFTSENLKLVHIVCHK KTTHKLNCDPSRIYKPQTRL KRKQPVRKRQ
Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Protein Name Dna Fragmentation Factor Subunit Beta
Caspase-Activated Deoxyribonuclease
Cad
Caspase-Activated Dnase
Caspase-Activated Nuclease
Cpan
Dna Fragmentation Factor 40 Kda Subunit
Dff-40
Database Links Reactome: R-HSA-140342
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Dna Fragmentation Factor Subunit Beta antibody
Anti-Caspase-Activated Deoxyribonuclease antibody
Anti-Cad antibody
Anti-Caspase-Activated Dnase antibody
Anti-Caspase-Activated Nuclease antibody
Anti-Cpan antibody
Anti-Dna Fragmentation Factor 40 Kda Subunit antibody
Anti-Dff-40 antibody
Anti-DFFB antibody
Anti-CAD antibody
Anti-DFF2 antibody
Anti-DFF40 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance