Immune System | Research Area | St John's Laboratory

Western Blot (WB): Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Annexin A1 STJ196742

Anti-ANXA1 antibody (250-346 aa) [ABT-ANXA1] (STJ196742)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£0.00

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Immunohistochemistry - Paraffin (IHC-P): Human esophagus tissue was stained with Anti-Annexin A1 (PT1761) antibody. STJ196943

Anti-ANXA1 antibody (250-346 aa) [PT1761] (STJ196943)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£0.00

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Western blot detection of Annexin A1 in A549, CHO-K1, COS7, C2C12, C6, 3T3 and Hela cell lysates using Annexin A1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Anti-ANXA1 antibody [5E4-D8-F12] (STJ99256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Anti-ANXA1 antibody [IHC512] (STJA0042008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

£131.50 - £350.50

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Anti-ANXA1 antibody [M021] (STJA0005256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£391.50

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Anti-ANXA1/Annexin A1 antibody [SAA1753] (STJA0020181)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Western blot analysis of extracts of various cell lines, using Annexin A2 rabbit monoclonal antibody (STJ11101300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ANXA2 antibody (1-100) [S0MR] (STJ11101300)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of NIH/3T3 cells using Annexin A2 Rabbit polyclonal antibody (STJ11103851) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ANXA2 antibody (1-339) (STJ11103851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Annexin II (PTR1321) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: NIH-3T3Predicted band size: 37kDaObserved band size: 37kDa

Anti-ANXA2 antibody (100-200) [PTR1321] (STJA0006280)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Annexin A2 antibody western blot C6 3T3 HeLa STJA0010957

Anti-ANXA2 antibody (33-46 aa) [R09-5C1] (STJA0010957)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various cells using Annexin II Polyclonal Antibody diluted at 1：2000

Anti-ANXA2 antibody (88-137 aa) (STJ91601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ANXA2 antibody (C-Term) [M298] (STJA0005258)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Chicken/Human/Mouse/Rat

£391.50

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Anti-ANXA2 antibody [M009] (STJA0005257)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£391.50

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Anti-ANXA2 antibody [SAA1563] (STJA0019991)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Immunofluorescence analysis of PC-12 cells using AOC1 Polyclonal Antibody (STJ28005) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AOC1 antibody (20-300) (STJ28005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AOC1 antibody (STJA0007550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£49.00 - £261.00

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Anti-AP-1 complex subunit beta-1 antibody (11-29 aa) (STJA0040955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ICC/IHC-F/IHC-P/WB

Reactivity :

Human/Rat

£540.50

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Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP1B1 antibody (215-265 aa) (STJ193661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using AP1B1 Rabbit polyclonal antibody (STJ118734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-AP1B1 antibody (700-800) (STJ118734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using AP1G1 Rabbit monoclonal antibody (STJ11103000) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-AP1G1 antibody (723-822) [S0MR] (STJ11103000)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-AP1G1 antibody (STJ192028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using AP1M1 antibody (STJ112168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AP1M1 antibody (1-160) (STJ112168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AP1M1 antibody (STJA0007179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP1M2 antibody (117-167 aa) (STJ194110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using AP1M2 Rabbit pAb (STJ110629) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 1s.

Anti-AP1M2 antibody (164-423) (STJ110629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AP1S3 antibody (STJA0004693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Anti-AP1S3 antibody (STJA0004694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Confocal imaging of NIH/3T3 cells using AP2A1 Rabbit monoclonal antibody (STJ11102071, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AP2A1 antibody (1-100) [S1MR] (STJ11102071)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using AP2A1 antibody (STJ28943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AP2A1 antibody (1-260) (STJ28943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AP2A1 antibody (STJA0008724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells using Alpha2-adaptin/AP2A2 Rabbit polyclonal antibody (STJ27344) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AP2A2 antibody (590-939) (STJ27344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Alpha2-adaptin/AP2A2 antibody (STJ11104629) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AP2A2 antibody (590-939) [S4629RM] (STJ11104629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-AP2A2 antibody (STJA0008820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP2B1 antibody (70-120 aa) (STJ195077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using AP2B1 Rabbit monoclonal antibody (STJ11105219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AP2B1 antibody (752-951 aa) [S5219RM] (STJ11105219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded rat brain using AP2B1 Rabbit polyclonal antibody (STJ22627) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-AP2B1 antibody (752-951) (STJ22627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP2M1 antibody (100-180 aa) (STJ190156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of HeLa cells, using AP2M1 antibody (STJ115897) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AP2M1 antibody (134-433) (STJ115897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using AP2M1 Rabbit polyclonal antibody (STJ22628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AP2M1 antibody (134-433) (STJ22628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunofluorescence analysis of U-251MG cells using AP2M1 Rabbit monoclonal antibody (STJ11101275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AP2M1 antibody (300-400) [S5MR] (STJ11101275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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AP2M1 antibody western blot HEK293 MCF-7 STJA0036095

Anti-AP2M1 antibody [R05-1C5] (STJA0036095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from Mouse brain, using AP2S1 Rabbit mAb (STJ11103654) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-AP2S1 antibody (1-100) [S4MR] (STJ11103654)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western blot analysis of various lysates using AP2S1 Rabbit polyclonal antibody (STJ114160) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-AP2S1 antibody (1-142) (STJ114160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-AP2S1 antibody (26-76 aa) (STJ194906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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AP2S1 antibody western blot SH-SY5Y 293T STJA0037487

Anti-AP2S1 antibody (STJA0037487)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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AP2S1 antibody western blot HeLa cells STJA0037050

Anti-AP2S1 antibody [R06-5T5] (STJA0037050)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of extracts of mouse testis, using APAF1 antibody (STJ117996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-APAF1 antibody (470-600) (STJ117996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of K562 cells using Apaf-1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-APAF1 antibody (501-550 aa) (STJ96743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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