Anti-ANXA2 antibody [M009] (STJA0005257)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenClone M009 was generated from a proprietary antigen related to human Annexin A2 in MDA-MB-231 breast cancer cell line.
General Information
| Short Description | Mouse monoclonal antibody anti-Annexin A2 is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Applications | WB/ICC/IP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M009 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Protein G Purified |
| Dilution Range | WB 1:1000ICC 1:100IP 1:100 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ANXA2 |
| Gene ID | 302 |
| Uniprot ID | ANXA2_HUMAN |
| Immunogen | Clone M009 was generated from a proprietary antigen related to human Annexin A2 in MDA-MB-231 breast cancer cell line. |
| Specificity | Clone M009 detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, HUVEC, and PC3 cells. The antibody also works for ELISA, immunocytochemical labeling, and immunoprecipitat |
Additional Info
| Post Translational Modifications | Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. |
| Function | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen. |
| Protein Name | Annexin A2Annexin IiAnnexin-2Calpactin I Heavy ChainCalpactin-1 Heavy ChainChromobindin-8Lipocortin IiPlacental Anticoagulant Protein IvPap-IvProtein IP36 |
| Database Links | Reactome: R-HSA-445355Reactome: R-HSA-6798695Reactome: R-HSA-75205Reactome: R-HSA-8950505 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneMelanosomeIn The Lamina Beneath The Plasma MembraneIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvTranslocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism |
| Alternative Antibody Names | Anti-Annexin A2 antibodyAnti-Annexin Ii antibodyAnti-Annexin-2 antibodyAnti-Calpactin I Heavy Chain antibodyAnti-Calpactin-1 Heavy Chain antibodyAnti-Chromobindin-8 antibodyAnti-Lipocortin Ii antibodyAnti-Placental Anticoagulant Protein Iv antibodyAnti-Pap-Iv antibodyAnti-Protein I antibodyAnti-P36 antibodyAnti-ANXA2 antibodyAnti-ANX2 antibodyAnti-ANX2L4 antibodyAnti-CAL1H antibodyAnti-LPC2D antibody |
Information sourced from Uniprot.org