• Western blot analysis of extracts of mouse brain cells, using AP2S1 antibody (STJ11103654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Mouse brain cells, using AP2S1 antibody (STJ11103654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AP2S1 antibody (1-100) [S4MR] (STJ11103654)

SKU:
STJ11103654

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AP2S1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AP2S1
Gene ID: 1175
Uniprot ID: AP2S1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AP2S1 (P53680).
Immunogen Sequence: MIRFILIQNRAGKTRLAKWY MQFDDDEKQKLIEEVHAVVT VRDAKHTNFVEFRNFKIIYR RYAGLYFCICVDVNDNNLAY LEAIHNFVEVLNEYFHNVCE
Function Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the ED-X-X-X-L-LI motif. May also play a role in extracellular calcium homeostasis.
Protein Name Ap-2 Complex Subunit Sigma
Adaptor Protein Complex Ap-2 Subunit Sigma
Adaptor-Related Protein Complex 2 Subunit Sigma
Clathrin Assembly Protein 2 Sigma Small Chain
Clathrin Coat Assembly Protein Ap17
Clathrin Coat-Associated Protein Ap17
Ha2 17 Kda Subunit
Plasma Membrane Adaptor Ap-2 17 Kda Protein
Sigma2-Adaptin
Database Links Reactome: R-HSA-167590
Reactome: R-HSA-177504
Reactome: R-HSA-182218
Reactome: R-HSA-2132295
Reactome: R-HSA-3928665
Reactome: R-HSA-416993 P53680-1
Reactome: R-HSA-437239
Reactome: R-HSA-5099900
Reactome: R-HSA-5140745
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8866427
Reactome: R-HSA-8964038
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Membrane
Coated Pit
Peripheral Membrane Protein
Cytoplasmic Side
Ap-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv
Alternative Antibody Names Anti-Ap-2 Complex Subunit Sigma antibody
Anti-Adaptor Protein Complex Ap-2 Subunit Sigma antibody
Anti-Adaptor-Related Protein Complex 2 Subunit Sigma antibody
Anti-Clathrin Assembly Protein 2 Sigma Small Chain antibody
Anti-Clathrin Coat Assembly Protein Ap17 antibody
Anti-Clathrin Coat-Associated Protein Ap17 antibody
Anti-Ha2 17 Kda Subunit antibody
Anti-Plasma Membrane Adaptor Ap-2 17 Kda Protein antibody
Anti-Sigma2-Adaptin antibody
Anti-AP2S1 antibody
Anti-AP17 antibody
Anti-CLAPS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance