Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates using ATF3 Rabbit polyclonal antibody (STJ22703) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced KitExposure time: 120 s.

Anti-ATF3 antibody (1-181) (STJ22703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of 293T-UV cells using ATF-3 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ATF3 antibody (131-180 aa) (STJ91752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from HeLa cells, using ATF4 Rabbit polyclonal antibody (STJ22704) at 1:1000 dilution. HeLa cells were treated by tunicamycin (2 Mu g/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ATF4 antibody (1-351) (STJ22704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Western blot analysis of A549 cells using CREB-2 Polyclonal Antibody

Anti-ATF4 antibody (160-240 aa) (STJ92467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of Hep G2 cells using ATF4 Rabbit polyclonal antibody (STJ11101481) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF4 antibody (260-351) (STJ11101481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

STJ70090 (1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATF4 antibody (C-Term) (STJ70090)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Pig

£312.50

Choose Options

Immunofluorescence analysis of U2OS cells using ATF5 Rabbit polyclonal antibody (STJ11100125) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATF5 antibody (101-282) (STJ11100125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human placenta using ATF5 Rabbit monoclonal antibody (STJ11101898) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-ATF5 antibody (183-282) [S8MR] (STJ11101898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using ATF5 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ATF5 antibody (207-224 aa) [R06-6G9] (STJA0010996)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£246.50

Choose Options

Western blot analysis of various cells using ATF-5 Polyclonal Antibody diluted at 1：1000

Anti-ATF5 antibody (221-270 aa) (STJ91753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

STJ70089 (1. 5µg/ml) staining of Human Heart lyssate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ATF5 antibody (C-Term) (STJ70089)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

Choose Options

Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ22705) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF6 antibody (190-670) (STJ22705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ATF6 antibody (221-271 aa) (STJ195942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

EB012080 (1µg/ml) staining of HeLa (A) and MCF7 (B) cell lysate, and HeLa (C) and Jurkat (D) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATF6 antibody (437-449) (STJ72994)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Rat/Dog/Pig/Cow

£312.50

Choose Options

Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF6 antibody (600-670) (STJ11103832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using ATF6B Rabbit polyclonal antibody (STJ119152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATF6B antibody (1-70) (STJ119152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various cells using ATF-6 Beta Polyclonal Antibody

Anti-ATF6B antibody (401-450 aa) (STJ91754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using ATF7 antibody (STJ29717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATF7 antibody (1-117) (STJ29717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Western blot analysis of various cells using ATF-7 Polyclonal Antibody

Anti-ATF7 antibody (41-90 aa) (STJ91755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

STJ70088 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATF7 antibody (C-Term) (STJ70088)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Rat/Dog/Cow

£312.50

Choose Options

Western blot analysis of extracts of various cell lines, using ATF7IP antibody (STJ116407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATF7IP antibody (525-725) (STJ116407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-ATF7IP antibody (STJA0008499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Anti-AtFIP37 antibody (STJ11106563)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

Choose Options

Anti-ATFS-1 antibody (STJ11106724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

C.elegans

£146.50 - £642.50

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using ATG10 Rabbit monoclonal antibody (STJ11102331) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG10 antibody (121-220) [S1MR] (STJ11102331)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded Rat testis using ATG10 Rabbit polyclonal antibody (STJ29527) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ATG10 antibody (204-220) (STJ29527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-ATG10 antibody (STJ13100527)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-ATG10 antibody (STJ13100533)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-ATG10 antibody (STJ13100539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-ATG10 antibody (STJ13100588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

Choose Options

Anti-ATG101 antibody (STJA0009720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of U2OS cells using ATG12 Rabbit polyclonal antibody (STJ118058) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG12 antibody (1-100) (STJ118058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-ATG12 antibody (20-70 aa) (STJ13100544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

D.melanogaster

£331.50

Choose Options

Western blot analysis of extracts of various cell lines, using ATG12 antibody (STJ11101729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ATG12 antibody (41-140) [S9MR] (STJ11101729)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Anti-Atg12 antibody at 1:500 dilution; 293HEK cells transfected with GFP-Atg12; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ATG12 antibody (65aa N-Term) (STJ140014)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

Choose Options

Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ATG12 antibody (80-130 aa) (STJ195672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-ATG12 antibody (STJ13100043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£331.50

Choose Options

Western blot analysis of 1) Hela Cell, 2) Rat Brain Tissue, 3) Mouse Brainr Tissue lysate using Rabbit pAb diluted at 1:2,000.

Anti-ATG13 antibody (182-232) (STJA0006722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-ATG13 antibody (281-480 aa) [SRM] (STJ11107161)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates, using ATG13 antibody (STJ22706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 25s.

Anti-ATG13 antibody (281-517) (STJ22706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of U2OS cells using ATG14 Rabbit polyclonal antibody (STJ29664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG14 antibody (340-440) (STJ29664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-ATG16L1 antibody (170-210 aa) (STJ13100026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

Choose Options

Immunofluorescence analysis of U2OS cells using ATG16L1 Rabbit polyclonal antibody (STJ113881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ATG16L1 antibody (200-300) (STJ113881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry of rat tissue with Anti-ATG16L1 primary antibody (STJ500180) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.

Anti-ATG16L1 antibody (560-610) (STJ500180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-ATG16L1 antibody (560-610) {Biotin} (STJ500181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-ATG16L1 antibody (560-610) {FITC} (STJ500182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit monoclonal antibody (STJ11101913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATG16L1 antibody (6-105) [S3MR] (STJ11101913)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

Choose Options

Western blot analysis of Jurkat using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG16L1 antibody (60-140 aa) (STJ91756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options