• Immunofluorescence analysis of HeLa cells using RhoGAP antibody (STJ22674) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RhoGAP antibody (STJ22674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ARHGAP5 antibody (1292-1501) (STJ22674)

SKU:
STJ22674

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARHGAP5 (1292-1501) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGAP5
Gene ID: 394
Uniprot ID: RHG05_HUMAN
Immunogen Region: 1292-1501
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human RhoGAP (NP_001164.2).
Immunogen Sequence: EGLYRVSGNKTDQDNIQKQF DQDHNINLVSMEVTVNAVAG ALKAFFADLPDPLIPYSLHP ELLEAAKIPDKTERLHALKE IVKKFHPVNYDVFRYVITHL NRVSQQHKINLMTADNLSIC FWPTLMRPDFENREFLSTTK IHQSVVETFIQQCQFFFYNG EIVETTNIVAPPPPSNPGQL VEPMVPLQLPPPLQPQLIQP QLQTDPLGII
Tissue Specificity Detected in skin fibroblasts (at protein level).
Function GTPase-activating protein for Rho family members.
Protein Name Rho Gtpase-Activating Protein 5
Rho-Type Gtpase-Activating Protein 5
P190-B
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013405
Reactome: R-HSA-9013406
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Also Membrane-Associated In A Fibrillar Pattern That Colocalizes With The Alpha5-Beta1 Integrin Receptor (Itga5/Itgb1) For Fibronectin
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 5 antibody
Anti-Rho-Type Gtpase-Activating Protein 5 antibody
Anti-P190-B antibody
Anti-ARHGAP5 antibody
Anti-RHOGAP5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance